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| Product Name | Advanced Fast 2-Step RT-qPCR SYBR Green Kit |
| Catalog No. | RESK-0081 |
| Description | A detection kit designed for reverse transcription and amplification of RNA templates rapidly released from cultured cell samples into lysis buffer. The kit includes a reverse transcription reagent (RT SuperMix) and a dye-based qPCR amplification reagent (2x qPCR SYBR Mix (ROX)). The system shows strong tolerance to lysate matrix and broad compatibility. The kit is suitable for high-throughput (96/384-well) analysis of cellular RNA expression. The 100 T format provides 96 x 2 qPCR reactions, enough to process one full 96-well cell plate in a single run, avoiding contamination and reagent performance loss caused by repeated opening and freeze-thaw. The 500 T format is intended for 384-well plates and higher-throughput automated screening platforms. |
| Intended Use | Reverse transcription and quantitative gene expression analysis of RNA released from cultured cell lysates, including high-throughput expression analysis in 96-well and 384-well formats. |
| Principle / Technology | Two-step (dye-based) RT-qPCR: reverse transcription and qPCR amplification are performed in two separate steps, with qPCR detection based on SYBR Green fluorescent dye binding to double-stranded DNA. |
| Detection Method | Dye-based real-time quantitative PCR (SYBR Green fluorescence detection) |
| Sample Type | RNA templates released from cultured cell samples into cell lysate |
| Performance Range / Specifications | The 100 T format provides 96 x 2 qPCR reactions, sufficient for one full 96-well plate Strong system tolerance to cell lysate matrix and broad compatibility Suitable for high-throughput 96/384-well cellular RNA expression analysis |
| Reaction Conditions / Protocol | 1. Reverse transcription: reverse transcribe the RNA template from cell lysate using the RT SuperMix 2. qPCR: perform dye-based qPCR amplification of the resulting cDNA with the 2x qPCR SYBR Mix (ROX) on a compatible real-time PCR instrument |
| Components / Formulation | RT SuperMix (reverse transcription reagent) 2x qPCR SYBR Mix (ROX) (dye-based qPCR amplification reagent) |
| Storage Conditions | Store at -25 to -15 °C |
| Shelf Life | 1 year |
| Package Specifications | 100 T; 500 T |
| Product Form | Liquid reagent kit (multi-component) |
| Expiration Date / Stability | Stable for 1 year when stored at -25 to -15 °C |
| Regulatory / Compliance | For research use only |
| Compatibility | The 2x qPCR SYBR Mix contains ROX reference dye; compatible with real-time PCR instruments requiring ROX normalization |
| Application Notes / Precautions | Process a full 96-well plate in a single run where possible to avoid contamination and reagent performance loss from repeated opening and freeze-thaw cycles The 500 T format is recommended for 384-well automated screening platforms |
For research use only, not for clinical use.
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