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| Product Name | 2x C51P1 TaqMan SNP Genotyping Master Mix |
| Catalog No. | RESK-0096 |
| Description | This product is a 2x concentrated fluorescent quantitative TaqMan SNP genotyping master mix built on a new-generation antibody-based hot-start Taq DNA polymerase. The genetically engineered hot-start Taq polymerase greatly improves amplification sensitivity and specificity, promoting efficient amplification of low-concentration templates. The product is suitable for genotyping and multiplex quantitative gene analysis. This formulation contains no ROX reference dye and is intended for instruments that do not require ROX normalization. |
| Intended Use | SNP genotyping and multiplex quantitative gene analysis by TaqMan probe-based real-time PCR; allelic discrimination assays. |
| Principle / Technology | Allele-specific TaqMan probe genotyping: two differentially fluorescently labeled hydrolysis probes, each perfectly matched to one allele, are combined with flanking PCR primers. During amplification, the 5' exonuclease activity of the hot-start Taq polymerase cleaves only the fully matched probe, generating an allele-specific fluorescent signal; the relative signal intensities of the two reporter dyes discriminate homozygous and heterozygous genotypes. The antibody-based hot-start mechanism suppresses non-specific priming and improves discrimination of low-abundance templates. |
| Detection Method | Real-time quantitative PCR with TaqMan allele-specific hydrolysis probes (endpoint or real-time allelic discrimination) |
| Sample Type | Genomic DNA and other DNA templates; not suitable for RNA templates. |
| Performance Range / Specifications | Suitable for genotyping and multiplex quantitative gene analysis; genetically engineered hot-start Taq polymerase supports efficient amplification of low-concentration templates. |
| Sensitivity / LOD | Genetically engineered hot-start Taq polymerase enables efficient amplification of low-concentration templates, improving detection sensitivity. |
| Specificity | Antibody-based hot-start Taq polymerase delivers high amplification specificity and reliable allele discrimination in TaqMan probe-based assays. |
| Reaction Conditions / Protocol | Standard TaqMan probe-based qPCR cycling with allele-specific probes and flanking primers; follow the thermal profile recommended for the assay and instrument. |
| Components / Formulation | 2x master mix containing genetically engineered antibody-based hot-start Taq DNA polymerase, reaction buffer, Mg2+ and dNTPs. ROX-free formulation (no passive reference dye). |
| Storage Conditions | Store at -25 to -15°C. |
| Shelf Life | 2 years when stored at -25 to -15°C. |
| Package Specifications | 1 mL |
| Product Form | Liquid 2x master mix |
| Concentration | 2x concentrated master mix |
| Expiration Date / Stability | Stable for 2 years at -25 to -15°C. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Designed for real-time PCR instruments that do not require a ROX reference dye; suitable for probe-based SNP detection on corresponding platforms. (A companion Low ROX formulation is available for instruments requiring Low ROX.) |
For research use only, not for clinical use.
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