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| Product Name | Urea, 8 M Solution, Molecular Biology Grade, Filtered |
| Catalog No. | ATR-SDS-0023 |
| Description | A ready-to-use 8 M urea solution in ultrapure water, deionized and filtered for protein denaturation, solubilization of inclusion bodies, and nucleic acid electrophoresis applications. This pre-dissolved format eliminates the endothermic dissolution process and associated preparation time, while ensuring consistent molarity across experiments. Each lot is tested for cyanate levels, which can carbamylate proteins at elevated temperatures. |
| Intended Use | Protein denaturation and solubilization of inclusion bodies; denaturing polyacrylamide gel electrophoresis (urea-PAGE); nucleic acid denaturation for Northern and Southern blotting; chaotropic disruption of protein aggregates. |
| Principle / Technology | Chaotropic agent that disrupts hydrogen bonding networks in proteins and nucleic acids, promoting unfolding and solubilization of aggregated biomolecules |
| Detection Method | Ready-to-use at 8 M; dilute as needed for specific applications; typical working concentration 2–8 M |
| Components / Formulation | Urea (CAS 57-13-6), 8 M (480.48 g/L) in ultrapure water; deionized with mixed-bed ion-exchange resin; 0.22 µm filtered |
| Storage Conditions | Store at 15–25 °C; protect from light; avoid heating above 30 °C to minimize cyanate formation |
| Shelf Life | 12 months from manufacture date; cyanate levels increase over time at elevated temperatures |
| Package Specifications | 100 mL, 500 mL, 1 L HDPE bottles; amber bottle option for light-sensitive applications |
| Product Form | Clear, colorless to very pale yellow solution |
| Key Features | Ready-to-use 8 M solution; deionized (conductivity <10 µS/cm); cyanate <0.1 mM at time of manufacture; 0.22 µm filtered; DNase/RNase-free tested; eliminates lengthy dissolution and deionization steps; consistent molarity lot-to-lot |
| Purity | Urea purity ≥99.5% by nitrogen content; cyanate <0.1 mM; heavy metals <5 ppm |
| Concentration | 8.0 ± 0.1 M; density approximately 1.13 g/mL at 20 °C |
| Activity / Unit Definition | Solubilization capacity as specified in product description |
| Molecular Weight | 60.06 g/mol (urea monomer) |
| Source / Origin | Synthetic, purified, and quality tested |
| pH Range / Optimal pH | Not applicable — organic solvent |
| Shipping Conditions | Ambient temperature; non-hazardous; protect from excessive heat during summer transport |
| Expiration Date / Stability | 12 months at 15–25 °C; cyanate content increases with time and temperature; do not use if solution develops ammonia odor indicating significant decomposition |
| Regulatory / Compliance | For laboratory and research use only; CAS 57-13-6; not classified as dangerous goods; USP/EP pharmacopoeia compliant for purity parameters |
| Compatibility | Compatible with glass, polypropylene, polyethylene, PTFE, and stainless steel; urea solutions will attack polycarbonate and some polyurethane formulations; avoid contact with nitrites which form explosive compounds |
| Recommended Buffer System | Use directly or dilute in compatible solvent as per protocol |
| Application Notes / Precautions | For inclusion body solubilization, use 8 M urea in appropriate buffer (e.g., 50 mM Tris, pH 8.0) with reducing agent (DTT or β-mercaptoethanol). For urea-PAGE, use 7–8 M urea in gel mix. Always prepare urea-containing buffers fresh; avoid heating above 30 °C. Cyanate can carbamylate lysine residues — include 20–50 mM ammonium bicarbonate to scavenge cyanate if needed. |
| Batch-to-Batch Consistency | Purity within ±0.5% of specification; water content within tolerance |
For research use only, not for clinical use.
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