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| Product Name | Triton X-100, 10% Aqueous Solution, Proteomics Grade, Filtered |
| Catalog No. | ATR-SDS-0027 |
| Description | Ready-to-use 10% w/v sterile-filtered aqueous solution of Triton X-100 (polyethylene glycol tert-octylphenyl ether), a nonionic surfactant commonly used for cell lysis, membrane protein solubilization, and permeabilization in biochemistry and cell biology applications. This proteomics-grade solution is pre-filtered through 0.1 µm membrane and tested for low peroxide and carbonyl content to minimize protein oxidation artifacts in mass spectrometry and western blotting workflows. The 10% concentration reduces handling of viscous neat Triton X-100, ensuring accurate pipetting and reproducible lysis conditions across experiments. |
| Intended Use | Cell lysis for protein extraction; membrane protein solubilization; cell permeabilization for immunofluorescence and intracellular staining; inclusion body solubilization; reduction of nonspecific binding in ELISA and western blot; emulsification of lipid samples. |
| Principle / Technology | Triton X-100 disrupts lipid-lipid and lipid-protein interactions in biological membranes while generally preserving protein-protein interactions; hydrophilic polyethylene oxide head group with hydrophobic tert-octylphenyl tail; critical micelle concentration (CMC) ~0.2 mM. |
| Detection Method | For cell lysis: add 10% stock to lysis buffer to achieve 0.1-1% final concentration; incubate on ice 15-30 minutes; centrifuge to collect soluble fraction. For permeabilization: use 0.1-0.5% in PBS for 5-15 minutes at RT. |
| Sample Type | Mammalian cells, bacterial cells, yeast cells, tissue homogenates, membrane preparations, inclusion bodies. |
| Performance Range / Specifications | Effective protein solubilization at 0.1-2% w/v; membrane protein extraction optimized at 0.5-1%; cell permeabilization at 0.05-0.5% depending on cell type; CMC ~0.015% w/v (0.24 mM). |
| Sensitivity / LOD | Peroxide content <1 mM in 10% solution as measured by ferrous oxidation-xylenol orange (FOX) assay; carbonyl content <50 nmol/mg. |
| Specificity | Nonionic surfactant prefers solubilization of membrane proteins; soluble cytoplasmic proteins generally extracted; protein-protein interactions typically preserved at ≤0.5% Triton X-100. |
| Reaction Conditions / Protocol | Lysis buffer preparation: add 10% Triton X-100 stock to chilled buffer with protease/phosphatase inhibitors; mix by gentle inversion; avoid vortexing to prevent foaming; use immediately or store at 2-8 °C. |
| Components / Formulation | Triton X-100 10% w/v in ultrapure water (18.2 MΩ·cm). |
| Storage Conditions | Store at room temperature (15-25 °C); protect from light to minimize peroxide formation. |
| Shelf Life | 24 months from date of manufacture in unopened container. |
| Package Specifications | 10 mL, 50 mL, 100 mL, 500 mL sterile bottles. |
| Product Form | Clear, colorless to pale yellow viscous liquid; mild characteristic odor. |
| Quality Control | Each lot tested for Triton X-100 concentration (refractive index), peroxide content (FOX assay), UV absorbance at 280 nm and 340 nm, pH, endotoxin, and sterility. |
| Key Features | Proteomics-grade, low peroxide and carbonyl; ready-to-use 10% solution for accurate pipetting; 0.1 µm sterile-filtered; reduced oxidation artifacts in MS and WB; nonionic and non-denaturing at moderate concentrations. |
| Purity | Triton X-100 ≥99% purity (peroxide-reduced grade); peroxide <1 mM; carbonyl <50 nmol/mg; UV A280 <0.05 (1% solution). |
| Concentration | 10% w/v in ultrapure water; typical working range 0.05-2% v/v. |
| Activity / Unit Definition | Protein extraction efficiency: ≥90% of total cellular protein solubilized at 1% Triton X-100; membrane protein yield 2-5 fold higher than mechanical lysis alone. |
| Molecular Weight | Triton X-100: average 625 g/mol (C14H22O(C2H4O)n, n=9-10); CMC ~0.2 mM. |
| Source / Origin | Synthetic from octylphenol ethoxylation; peroxide-reduced grade via proprietary purification process. |
| pH Range / Optimal pH | 6.0-8.0 in water; aqueous solutions have near-neutral pH. |
| Shipping Conditions | Ambient temperature; protect from light. |
| Expiration Date / Stability | 24 months at room temperature unopened; after opening, use within 12 months. Peroxide content slowly increases over time. |
| Regulatory / Compliance | For research use only; not for diagnostic or therapeutic applications. Contains octylphenol ethoxylate — dispose according to local environmental regulations. |
| Compatibility | Compatible with Tris, HEPES, PBS, and most standard biochemical buffers. Compatible with BCA protein assay at ≤0.1% final concentration. Interferes with Bradford assay at >0.01%. Removable by dialysis or detergent removal columns. Compatible with trypsin digestion for MS after appropriate clean-up. |
| Recommended Buffer System | Not applicable — 10% aqueous solution; add to desired buffer at working concentration. |
| Application Notes / Precautions | Store stock solution in the dark at room temperature. Do not autoclave — autoclaving accelerates peroxide formation. For MS proteomics, use fresh bottle and purge headspace with nitrogen before reclosing. For membrane protein extraction, include protease inhibitors and work at 4 °C. For permeabilization of fixed cells, Triton X-100 at 0.1-0.2% for 10 minutes is typical. For nuclear envelope permeabilization, higher concentrations (0.5%) may be needed. Monitor peroxide levels in opened bottles older than 6 months. |
| Batch-to-Batch Consistency | Triton X-100 concentration within ±5% of specification; peroxide <1 mM; UV absorbance at 280 nm <0.05 AU. |
For research use only, not for clinical use.
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