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| Product Name | SYBR Safe DNA Gel Stain, 10,000x Concentrate in DMSO |
| Catalog No. | ATRSA-0005 |
| Description | A safer nucleic acid gel stain with reduced mutagenicity compared to ethidium bromide, providing high sensitivity detection of double-stranded DNA in agarose and polyacrylamide gels under blue light or UV transillumination. |
| Intended Use | Fluorescent staining of DNA in agarose gels for visualization and documentation of PCR products, restriction digests, and other DNA analysis applications in molecular biology laboratories. |
| Principle / Technology | SYBR Safe intercalates between DNA base pairs and exhibits a >1000-fold fluorescence enhancement upon binding; excitation maxima at 280 and 502 nm enable both UV and blue light visualization. |
| Detection Method | Fluorescence imaging with UV transilluminator (302 nm) or blue light transilluminator (470 nm), compatible with standard gel documentation systems |
| Sample Type | DNA fragments, PCR products, plasmid DNA, genomic DNA digests in agarose gels |
| Performance Range / Specifications | Detection limit ~0.5 ng DNA per band (comparable to ethidium bromide); linear signal up to 50 ng/band |
| Sensitivity / LOD | ~0.5 ng per band for double-stranded DNA under blue light excitation |
| Specificity | Preferentially stains double-stranded DNA; also stains single-stranded DNA and RNA with lower sensitivity |
| Reaction Conditions / Protocol | Dilute 1:10,000 in gel before pouring or in TAE/TBE running buffer; alternatively, post-stain gel in 1:10,000 dilution for 30 min. |
| Components / Formulation | SYBR Safe cyanine dye in DMSO at 10,000x concentration |
| Storage Conditions | 15–30°C, protect from light |
| Shelf Life | 12 months after opening |
| Package Specifications | 400 µL (for 4 L gel), 1 mL |
| Product Form | Liquid concentrate in DMSO |
| Quality Control | Sensitivity and linearity testing with DNA ladder, mutagenicity testing (AMES test negative), lot-to-lot consistency |
| Key Features | AMES test negative for mutagenicity; compatible with blue light and UV; ready-to-use concentrate; sensitive detection; safer alternative to ethidium bromide |
For research use only, not for clinical use.
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