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| Product Name | Hematoxylin and Eosin Staining Kit, Progressive Method |
| Catalog No. | ATRSA-0003 |
| Description | A complete H&E staining kit containing Gill's hematoxylin, eosin Y, bluing reagent, and clearing agent for routine histological staining of formalin-fixed paraffin-embedded tissue sections. |
| Intended Use | Routine histological examination of FFPE tissue sections for morphological assessment, pathological diagnosis, and research histology in clinical and research pathology laboratories. |
| Principle / Technology | Hematoxylin (oxidized to hematein with aluminum mordant) stains basophilic structures (nuclei) blue-purple through binding to DNA; eosin Y stains eosinophilic structures (cytoplasm, collagen, muscle) pink through electrostatic interactions. |
| Detection Method | Bright-field light microscopy, standard histological examination |
| Sample Type | Formalin-fixed paraffin-embedded tissue sections (3–5 µm on glass slides) |
| Performance Range / Specifications | Nuclear detail clearly resolved at 10x–40x magnification; cytoplasmic and extracellular matrix differentiation distinct |
| Sensitivity / LOD | N/A |
| Specificity | Nuclei stained blue-purple; cytoplasm and extracellular matrix stained pink in varying intensities by tissue type |
| Reaction Conditions / Protocol | Deparaffinize and rehydrate slides; stain in hematoxylin for 3–5 min; rinse; blue in alkaline solution for 30 sec; counterstain in eosin for 30 sec–2 min; dehydrate, clear, and mount. |
| Components / Formulation | Gill's Hematoxylin No. 2, Eosin Y alcoholic solution, Bluing Reagent (Scott's tap water substitute), xylene substitute clearing agent |
| Storage Conditions | 15–30°C, tightly sealed, protect from light |
| Shelf Life | 18 months |
| Package Specifications | Kit for 500 slides, 1000 slides |
| Product Form | Liquid reagents in kit format |
| Quality Control | Staining quality validation with multi-tissue control block, lot release testing with reference pathologist review |
| Key Features | Complete kit with all reagents; progressive method for easy control of staining intensity; validated for automated stainers; consistent lot-to-lot quality |
For research use only, not for clinical use.
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