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| Product Name | Nigrosin Negative Stain for Bacterial Capsules and Spores |
| Catalog No. | ATRSA-0016 |
| Description | A 10% aqueous nigrosin solution for negative staining of bacterial capsules and spores, providing a dark background against which unstained structures appear as clear halos or bright refractile bodies. |
| Intended Use | Demonstration of bacterial capsules (Klebsiella pneumoniae, Streptococcus pneumoniae, Cryptococcus neoformans) and endospores (Bacillus, Clostridium species) by negative staining in microbiology teaching and clinical laboratories. |
| Principle / Technology | Nigrosin, an acidic aniline dye, does not penetrate bacterial cells or capsules due to charge repulsion; it forms a dark background film against which the unstained capsule appears as a clear zone and spores as bright refractile oval bodies. |
| Detection Method | Bright-field light microscopy at 100x oil immersion |
| Sample Type | Bacterial cultures (capsule-producing strains), spore-forming bacteria, fungal cultures (Cryptococcus) |
| Performance Range / Specifications | Capsules appear as distinct clear halos 1–2 µm around bacterial cells; spores visible as unstained bright ovals within dark background |
| Sensitivity / LOD | N/A |
| Specificity | Negative staining technique; capsules and spores unstained against dark background |
| Reaction Conditions / Protocol | Mix a loopful of culture with a drop of nigrosin on a slide; spread thinly with another slide edge; air dry without heat fixation; examine under oil immersion. |
| Components / Formulation | Nigrosin (water-soluble aniline black) 10% w/v in deionized water with 0.5% formalin as preservative |
| Storage Conditions | 15–30°C, tightly sealed to prevent evaporation |
| Shelf Life | 24 months |
| Package Specifications | 100 mL dropper bottle |
| Product Form | Liquid, ready to use |
| Quality Control | Negative staining quality with K. pneumoniae (capsule positive) and E. coli (control), staining film uniformity |
| Key Features | Ready to use; no heat fixation needed; simple technique; clearly demonstrates capsules and spores; economical |
For research use only, not for clinical use.
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