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| Product Name | Modified Wright-Giemsa Stain for Blood Smears |
| Catalog No. | ATRSA-0019 |
| Description | A combination Wright-Giemsa staining solution optimized for the differential staining of peripheral blood and bone marrow smears, providing excellent nuclear and cytoplasmic detail for hematological evaluation. |
| Intended Use | Differential staining of blood cells in peripheral blood smears and bone marrow aspirates for morphological examination, white blood cell differential counting, and detection of hematological abnormalities. |
| Principle / Technology | Wright's stain (methylene blue eosinate) and Giemsa stain (azure dyes and methylene blue) together provide differential staining of cellular components: acidic structures (DNA, RNA) stain blue-purple with basic dyes, while basic proteins (hemoglobin, eosinophil granules) stain pink-orange with acidic eosin. |
| Detection Method | Bright-field light microscopy at 50× and 100× oil immersion magnification |
| Sample Type | Methanol-fixed peripheral blood smears or bone marrow aspirate smears on glass slides |
| Performance Range / Specifications | Staining protocol: 3–5 minutes total contact time; optimal staining time may vary with laboratory conditions and slide thickness |
| Sensitivity / LOD | Morphological detail sufficient for identification of all normal peripheral blood cell types and most abnormal cells |
| Specificity | Differential staining of erythrocytes (pink), lymphocyte nuclei (dark purple), neutrophil granules (pale pink), eosinophil granules (orange-red), basophil granules (dark blue-purple), and platelet granules (purple) |
| Reaction Conditions / Protocol | Fix air-dried blood smear in absolute methanol for 1 minute, flood slide with Wright-Giemsa working solution for 3 minutes, add phosphate buffer (pH 6.8) and allow to stand for 5–7 minutes, rinse gently with buffer, air-dry, and examine microscopically |
| Components / Formulation | Wright-Giemsa stain concentrate, phosphate buffer concentrate (pH 6.8), methanol fixative, coplin staining jar, detailed protocol with troubleshooting guide |
| Storage Conditions | Stain concentrate at room temperature protected from light; methanol fixative in a flammable storage cabinet at room temperature |
| Shelf Life | 24 months for stain concentrate |
| Package Specifications | 500 mL stain concentrate (sufficient for approximately 2500 slides when prepared as working solution) |
| Product Form | Liquid stain concentrate requiring dilution before use |
| Quality Control | Each lot tested on normal and abnormal blood smears with Wright-Giemsa staining characteristics verified by a certified medical technologist; dye content analyzed by spectrophotometry |
| Key Features | Combined Wright-Giemsa formulation provides both the crisp nuclear detail of Wright stain and the enhanced cytoplasmic granule staining of Giemsa; standardized buffer pH optimizes staining consistency across different blood smear preparations |
For research use only, not for clinical use.
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