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| Product Name | Papanicolaou Stain Kit, for Cervical Cytology |
| Catalog No. | ATRSA-0013 |
| Description | A complete Pap stain kit following the standard Papanicolaou staining protocol for the differential staining of cellular components in gynecological and non-gynecological cytology specimens. |
| Intended Use | Routine staining of cervical-vaginal smears (Pap test), fine needle aspiration specimens, body cavity fluids, and urinary cytology preparations for cancer screening and diagnostic cytopathology. |
| Principle / Technology | Hematoxylin stains nuclei; OG-6 counterstain highlights keratin and mature squamous cells orange; EA polychrome stain (eosin, light green, Bismarck brown) differentiates cytoplasm of squamous cells, glandular cells, and inflammatory cells. |
| Detection Method | Bright-field light microscopy |
| Sample Type | Cervical-vaginal smears (conventional and liquid-based), FNA slides, effusion cytology, urine cytology, brush cytology |
| Performance Range / Specifications | Nuclear chromatin detail clearly resolved; cytoplasmic staining differentiates squamous, glandular, and metaplastic cells |
| Sensitivity / LOD | N/A |
| Specificity | Nuclei: blue-black; superficial squamous cells: pink-orange; intermediate/parabasal cells: blue-green; glandular cells: blue-green granular |
| Reaction Conditions / Protocol | Fix smears in 95% ethanol immediately; hydrate through graded alcohols; hematoxylin 5 min; rinse; OG-6 1.5 min; rinse in 95% ethanol; EA-50 2.5 min; dehydrate in absolute ethanol; clear in xylene; mount. |
| Components / Formulation | Gill's Hematoxylin, OG-6 (Orange G in phosphotungstic acid), EA-50 (Eosin Y, Light Green SF, Bismarck Brown Y in ethanol), ethanol grades not included |
| Storage Conditions | 15–30°C, tightly sealed, protect from light, OG-6 and EA at 2–8°C to extend stability |
| Shelf Life | 12 months |
| Package Specifications | Kit for 500 slides |
| Product Form | Liquid reagents |
| Quality Control | Staining quality validation with reference cervical smear slides, cytoplasmic color differentiation verification |
| Key Features | Standard for cytology; distinct cytoplasmic coloration; ready to use; validated for conventional and liquid-based cytology; compatible with automated stainers |
For research use only, not for clinical use.
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