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Cat.No: FS-1648 Datasheet Instruction for Use
Specification Quantities
Price $445.59–742.65
| Product Name | Sperm Vitality Kit (Eosin-Nigrosin Method) |
| Catalog No. | FS-1648 |
| Description | The sperm vitality staining solution (eosin-aniline black method) can routinely assess the survival rate of all sperm specimens. It identifies sperm with intact cell membranes using the dye-rejection principle, thereby determining the percentage of live sperm. This staining solution consists of eosin and aniline black dyes and utilizes the principle of dye resistance. Specifically, damaged cell membranes—such as those found on non-viable (dead) cells—allow non-membrane-permeable dyes to penetrate and stain the interior; in contrast, the cell membranes of viable cells resist dye penetration, resulting in a lack of staining. |
| Test Procedure | Place the fresh semen sample in a 37°C incubator or water bath for 30 minutes to allow the sperm to fully liquefy, then gently swirl to mix. Take a clean small test tube, add 1 drop (5–10 µL) of fresh semen and an equal volume of eosin staining solution, mix well, and let stand for 15 seconds. Add 2 drops (10–20 µL) of aniline black staining solution, mix well to create the semen-eosin-aniline black mixture, and let stand for 15–30 seconds. Place 1 drop (5–10 µL) of the prepared semen-eosin-aniline black mixture onto a microscope slide to prepare a smear. Allow to air-dry and examine under a microscope. Under oil immersion, unstained sperm appear white, dead sperm appear red, and the background appears purplish-red. Count 200 sperm and calculate the percentage of unstained (live) sperm out of the 200 sperm. |
| Results | Live sperm—unstained Dead sperm—red Background—purple-red |
| Storage and Transportation | Store at room temperature, protected from light. Shelf life: 1 year. |
| Precautions | Once the semen sample has liquefied, the sperm survival rate should be tested immediately, preferably within 30 minutes, and under no circumstances should this exceed 1 hour. This is to prevent inaccurate staining results caused by sperm inactivation due to dehydration or temperature changes. A small amount of salt crystals may be observed after the smear dries; this is normal. |
For research use only, not for clinical use.
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