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Cat.No: FS-1649 Datasheet Instruction for Use
Specification Quantities
Price $397.05–661.75
| Product Name | Sperm Vitality Detection Solution (Eosin Method) |
| Catalog No. | FS-1649 |
| Description | Eosin is a synthetic azo dye that appears as a peach-colored or pink powder. Its molecular formula is C20H6O5Br4Na2, and its molecular weight is 691.86. Clinically, sperm viability can be assessed by testing the integrity of the sperm membrane. Sperm viability staining solution (the eosin method) can be used to routinely test the viability of all sperm specimens. By applying the dye-rejection method to identify sperm with intact cell membranes, the percentage of live sperm can be determined. This staining solution consists of eosin, deionized water, and other components. It utilizes the principle of dye resistance, whereby damaged cell membranes—such as those on non-viable (dead) cells—allow non-membrane-permeable dyes to penetrate and stain the interior; in contrast, the cell membranes of viable cells resist dye penetration, resulting in a resistance to staining and thus remaining unstained. This staining solution is intended for research purposes only and is not suitable for clinical diagnosis or other applications. |
| Test Procedure | Take a clean microscope slide, add one drop each of fresh semen and sperm vital stain (eosin method), and mix thoroughly. Cover with a coverslip, let stand for 30–60 seconds, and immediately examine under high magnification (e.g., 400×). Count 200 sperm and calculate the percentage of unstained (live) sperm out of the total 200. |
| Results | Live sperm—unstained Dead sperm—red |
| Storage and Transportation | Store at room temperature, protected from light. Shelf life: 1 year. |
| Precautions | Once the semen sample has liquefied, the sperm viability should be tested immediately; under no circumstances should this exceed 1 hour, to prevent inaccurate staining results caused by sperm inactivation due to dehydration or temperature fluctuations. For your safety and health, please wear a lab coat and disposable gloves when performing this procedure. |
For research use only, not for clinical use.
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