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| Product Name | Sodium Dodecyl Sulfate, 10% Solution, Molecular Biology Grade |
| Catalog No. | ATR-SDS-0022 |
| Description | A 10% (w/v) aqueous solution of sodium dodecyl sulfate (SDS), the anionic detergent essential for SDS-PAGE, nucleic acid extraction, and protein denaturation protocols. This pre-dissolved format eliminates the respiratory hazard of SDS powder handling and provides precise, reproducible concentrations. Each lot is tested for purity by UV spectrophotometry and confirmed free of DNase and RNase activities. |
| Intended Use | Protein denaturation for SDS-PAGE; nucleic acid extraction and purification; cell lysis; hybridization buffer component; bacterial alkaline lysis plasmid preparation; CMC determination studies. |
| Principle / Technology | Anionic detergent that binds to proteins at a constant ratio (1.4 g SDS per g protein), imparting negative charge proportional to molecular weight and denaturing secondary and tertiary structure |
| Detection Method | Typical working concentration: 0.1–2.0% (w/v); add to buffers and gels as specified in individual protocols |
| Components / Formulation | Sodium dodecyl sulfate (CAS 151-21-3), 10% (w/v) in ultrapure water; 0.22 µm filtered |
| Storage Conditions | Store at 15–25 °C; protect from light; SDS may precipitate below 15 °C — warm to room temperature and mix before use |
| Shelf Life | 24 months from manufacture date |
| Package Specifications | 100 mL, 500 mL, 1 L HDPE bottles |
| Product Form | Clear, colorless to pale yellow, moderately viscous liquid; may form precipitate at low temperatures (reversible upon warming) |
| Key Features | Molecular biology grade; DNase/RNase-free tested; A260/A280 tested for nucleic acid contamination; pre-filtered 0.22 µm; eliminates SDS dust inhalation hazard; consistent 10.0 ± 0.3% (w/v) concentration; low metal content for enzymatic applications |
| Purity | SDS 10.0 ± 0.3% (w/v); purity of SDS ≥99% by GC after lyophilization; alkyl sulfate composition meets electrophoresis grade specifications |
| Concentration | 10% (w/v); 10 g SDS per 100 mL final volume |
| Activity / Unit Definition | Solubilization capacity as specified in product description |
| Molecular Weight | As specified in product documentation |
| Source / Origin | Synthetic, purified, and quality tested |
| pH Range / Optimal pH | pH 6.0–7.5 (neat solution) |
| Shipping Conditions | Ambient temperature; non-hazardous; protect from freezing which causes irreversible precipitation in solution form |
| Expiration Date / Stability | 24 months at 15–25 °C; if precipitation occurs due to low temperature, warm to 30–37 °C and mix gently until clear; do not use if precipitate does not redissolve |
| Regulatory / Compliance | For laboratory and research use only; CAS 151-21-3; GHS07 classified (powder form); solution form not classified as dangerous goods |
| Compatibility | Compatible with glass, polypropylene, polyethylene, and PTFE; avoid contact with strong acids which regenerate dodecyl sulfate; SDS is incompatible with potassium salts (forms insoluble potassium dodecyl sulfate precipitate) |
| Recommended Buffer System | Use directly or dilute in compatible solvent as per protocol |
| Application Notes / Precautions | For SDS-PAGE sample preparation, add to loading buffer to 2% final concentration. For nucleic acid extraction, use 0.5–1.0% SDS in lysis buffer. Warm solution to 25–30 °C if precipitation is observed; do not autoclave SDS solutions. SDS-containing samples should be heated at 95 °C for 5 minutes before SDS-PAGE loading. |
| Batch-to-Batch Consistency | Purity within ±0.5% of specification; water content within tolerance |
For research use only, not for clinical use.
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