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| Product Name | Pyruvate Kinase Assay Reagent |
| Catalog No. | CMTR-HMM-0004 |
| Description | Pyruvate kinase (PK, EC 2.7.1.40) is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, producing one molecule of pyruvate and one molecule of ATP. A deficiency in pyruvate kinase slows down the glycolytic process, leading to pyruvate kinase deficiency. |
| Application | This product is used to measure pyruvate kinase activity in cell culture supernatants, plasma, serum, tissues, urine, and other biological fluids (not yet validated for use with whole blood). |
| Principle | PEP and ADP are catalyzed by pyruvate kinase to form pyruvate and ATP. The generated pyruvate is oxidized by pyruvate oxidase, producing color (absorbance at 570 nm) and fluorescence (excitation/emission wavelengths at 535/587 nm). Since the increase in color or fluorescence intensity is directly proportional to the amount of pyruvate, PK activity can be accurately measured. |
| Synonyms | CTHBP; EC 2.7.1.40; MGC3932 |
| Assay Type | Quantitative |
| Detection Method | Colorimetric; Fluorometric |
| Applicable Instruments | Microplate reader |
| Detection Time | 40 min |
| Sample Type | Adherent cells, Cell culture supernatant, Other biological fluids, Serum, Plasma, Suspension cells, Tissue |
| Detection Limit | 0.1 mU/mL |
| Procedure | 1. Add the sample and standard to the wells. 2. Add the reaction mixture. 3. Analyze immediately using a microplate reader, and analyze again after 10-20 minutes. |
| Storage | -20°C |
For research use only, not for clinical use.
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