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| Product Name | L-Lactate Assay Reagent |
| Catalog No. | CMTR-HMM-0025 |
| Description | Lactic acid (CH₃CH(OH)COO⁻) plays a crucial role in many biological processes. Abnormally elevated lactic acid concentrations are associated with conditions such as diabetes and lactic acidosis. |
| Application | This product is used to detect L(+)-lactic acid concentration in biological samples such as blood, cells, culture medium, and fermentation medium. |
| Principle | Lactic acid reacts specifically with an enzyme mixture to produce a product that interacts with the lactic acid probe to generate color (570 nm) and fluorescence (Ex/Em = 535/587 nm). |
| Assay Type | Quantitative |
| Detection Method | Colorimetric; Fluorometric |
| Applicable Instruments | Microplate reader |
| Detection Time | 40 min |
| Sample Type | Cell culture supernatant, Other biological fluids, Cell lysate, Serum, Plasma, Tissue lysate, Urine |
| Detection Limit | 0.001 mM |
| Standard Linear Range | 0.001 mM - 10 mM |
| Procedure | 1. Add the sample (deproteinized) and standard to the wells. 2. Add the reaction mixture and incubate at room temperature for 30 minutes. 3. Analyze using a microplate reader. |
| Storage | -20°C |
For research use only, not for clinical use.
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