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L-Lactate Assay Reagent

Cat.No: CMTR-HMM-0025 Datasheet

Specification Quantities

100T:
- +
2000T:
- +
Product Details Related Products
Product Name L-Lactate Assay Reagent
Catalog No. CMTR-HMM-0025
Description Lactic acid (CH₃CH(OH)COO⁻) plays a crucial role in many biological processes. Abnormally elevated lactic acid concentrations are associated with conditions such as diabetes and lactic acidosis.
Application This product is used to detect L(+)-lactic acid concentration in biological samples such as blood, cells, culture medium, and fermentation medium.
Principle Lactic acid reacts specifically with an enzyme mixture to produce a product that interacts with the lactic acid probe to generate color (570 nm) and fluorescence (Ex/Em = 535/587 nm).
Assay Type Quantitative
Detection Method Colorimetric; Fluorometric
Applicable Instruments Microplate reader
Detection Time 40 min
Sample Type Cell culture supernatant, Other biological fluids, Cell lysate, Serum, Plasma, Tissue lysate, Urine
Detection Limit 0.001 mM
Standard Linear Range 0.001 mM - 10 mM
Procedure 1. Add the sample (deproteinized) and standard to the wells. 2. Add the reaction mixture and incubate at room temperature for 30 minutes. 3. Analyze using a microplate reader.
Storage -20°C

For research use only, not for clinical use.

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