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| Product Name | 2 Hydroxyglutarate Assay Reagent |
| Catalog No. | CMTR-HMM-0036 |
| Description | In eukaryotic cells, isocitrate dehydrogenase (IDH1, IDH2, and IDH3) catalyzes the interconversion of isocitrate and α-ketoglutarate. In human cancers, IDH mutations result in gain-of-function alterations, reducing their affinity for isocitrate and promoting the conversion of α-ketoglutarate to D-2-hydroxyglutarate in the presence of NADP. D-2-hydroxyglutarate (D2HG, D-2-hydroxyglutarate) is present at low levels in normal cells and tissues but is significantly elevated in metabolic disorders and various cancers. Therefore, detecting elevated D2HG is crucial for the early diagnosis, prognosis, and development of treatment strategies for these diseases. |
| Application | This product is used to detect D-2-hydroxyglutaric acid (D2HG) in urine, cells, and tissue lysates. |
| Principle | In the presence of D2HG enzyme and substrate mixture, D-2-hydroxyglutaric acid is oxidized to α-ketoglutaric acid. The intermediate reduces the probe to a colored product with strong absorbance at OD 450 nm. This absorbance is directly proportional to the D2HG content in the sample. |
| Assay Type | Quantitative |
| Detection Method | Colorimetric |
| Applicable Instruments | Microplate reader |
| Sample Type | Cell lysate, Serum, Plasma, Tissue lysate, Urine |
| Detection Limit | 10 µM |
| Storage | -20°C |
For research use only, not for clinical use.
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