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| Product Name | Glucose Assay Kit (Fluorometric, Hexokinase-Coupled) |
| Catalog No. | CMTR-HMM-0070 |
| Description | Fluorometric assay for quantitative glucose measurement in cell culture supernatants, lysates, serum, and plasma using hexokinase-coupled enzymatic detection. The hexokinase reaction specifically phosphorylates glucose, with subsequent NADH-driven fluorogenic probe reduction providing a signal proportional to glucose concentration. Minimal interference from reducing substances commonly found in biological specimens. |
| Intended Use | Glucose quantification for metabolic flux analysis, cellular glucose uptake studies, diabetes research, and glycolysis rate measurement in cell culture models. |
| Principle / Technology | Hexokinase phosphorylates glucose to glucose-6-phosphate; G6PDH oxidizes G6P with NAD+ reduction to NADH; NADH reduces fluorogenic probe via enzymatic cycling (Ex/Em 535/587 nm) |
| Detection Method | Fluorometric; excitation 530-540 nm, emission 585-595 nm; also readable by absorbance at 570 nm |
| Sample Type | Cell culture supernatant, cell lysates, serum, plasma, urine; 2-20 µL per assay |
| Sensitivity / LOD | Detection range 0.2-50 µM in assay well; LOD 0.1 µM glucose |
| Specificity | Highly specific for D-glucose; no cross-reactivity with galactose, fructose, mannose, or sucrose |
| Reaction Conditions / Protocol | Add sample to plate with assay buffer; add hexokinase/G6PDH reaction mix; incubate 30 min at 37 °C; read fluorescence |
| Components / Formulation | Hexokinase/G6PDH mix, NAD+ cofactor, fluorogenic probe (in DMSO), glucose standard (1.0 mM), assay buffer |
| Storage Conditions | Store at -20 °C; protect probe from light; glucose standard at 4 °C |
| Shelf Life | 12 months from manufacture date |
| Package Specifications | 100 assays, 500 assays in 96-well format |
| Product Form | Lyophilized enzymes with liquid buffers |
| Key Features | Hexokinase coupling ensures glucose specificity; 50× more sensitive than colorimetric methods; minimal reducing substance interference; linear standard curve R² >0.995; high-throughput compatible |
| Purity | Hexokinase >150 U/mg; G6PDH >200 U/mg; glucose standard purity 99.7% |
| Concentration | As specified per kit format; probe stock solutions in DMSO as specified |
| Activity / Unit Definition | Standard curve linearity R² ≥0.99 per lot; dynamic range as specified |
| Molecular Weight | Varies by probe and buffer component |
| Source / Origin | Synthetic fluorescent probes; recombinant enzymes; purified metabolite standards |
| pH Range / Optimal pH | pH 7.2–7.4 for cell-based assays; pH varies for biochemical metabolite detection |
| Shipping Conditions | Cold pack -20 °C; protect fluorogenic probe from light |
| Expiration Date / Stability | 12 months at -20 °C; reconstituted enzymes stable 2 weeks at 4 °C |
| Regulatory / Compliance | For laboratory and research use only; RUO; ISO 9001 manufactured |
| Compatibility | Compatible with fluorescence microplate readers, luminometers, and spectrophotometers |
| Recommended Buffer System | Assay-specific buffer optimized per product protocol |
| Application Notes / Precautions | Normalize signal to cell number or protein content; avoid probe photobleaching; include appropriate antioxidant controls for ROS assays |
| Batch-to-Batch Consistency | Standard curve parameters within ±15% of reference lot |
For research use only, not for clinical use.
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