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L-Carnitine Assay Reagent

Cat.No: CMTR-HMM-0039 Datasheet

Specification Quantities

100T:
- +
Product Details Related Products
Product Name L-Carnitine Assay Reagent
Catalog No. CMTR-HMM-0039
Description Carnitine is a quaternary ammonium compound biosynthesized from the amino acids lysine and methionine. It participates in the transport of fatty acids into the mitochondrial matrix via the carnitine/acylcarnitine shuttle, where β-oxidation occurs to generate acetate, which then enters the TCA cycle to produce energy. L-carnitine is typically sold as a dietary supplement. Carnitine has two stereoisomers, but only L-carnitine possesses biological activity.
Application This product is used for detecting free L-carnitine in biological samples (e.g., serum).
Principle The transfer of the acetyl group from CoA to carnitine results in the formation of free CoA, which further reacts with the OxiRed probe in an oxidative reaction, producing fluorescence (Ex/Em 535 nm, 587 nm) and absorbance (570 nm).
Synonyms Carnitine; L Carnitine
Assay Type Quantitative
Detection Method Colorimetric; Fluorometric
Applicable Instruments Microplate reader
Detection Time 30 min
Sample Type Other biological fluids, Cell lysate, Serum, Plasma, Tissue lysate, Urine
Standard Linear Range 1 µM - 200 µM
Storage -20°C

For research use only, not for clinical use.

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