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| Product Name | Pyruvate Assay Reagent |
| Catalog No. | CMTR-HMM-0003 |
| Description | Pyruvate is a key cross-molecule in metabolic pathway networks. Pyruvate can be produced from glucose through glycolysis, converted back into carbohydrates via gluconeogenesis, or converted into fatty acids or energy via acetyl-CoA. It can also be used to synthesize the amino acid alanine and converted into ethanol. Glucose is converted into pyruvate, which is then converted into acetyl-CoA, the primary input for the citric acid cycle (TCA). If oxygen is insufficient, pyruvate undergoes anaerobic decomposition, producing lactic acid in animals and ethanol in plants and microorganisms. |
| Application | This product is used to measure pyruvate concentrations in various biological samples, including blood, cells, cultures, and fermentation media. |
| Principle | Pyruvate is oxidized by pyruvate oxidase through an enzymatic reaction, producing color (λ = 570 nm) and fluorescence (Ex/Em = 535/587 nm). Since the intensity of color or fluorescence is directly proportional to pyruvate concentration, pyruvate concentration can be accurately determined. |
| Assay Type | Quantitative |
| Detection Method | Colorimetric; Fluorometric |
| Applicable Instruments | Microplate reader |
| Detection Time | 40 min |
| Sample Type | Cell culture extracts, Other biological fluids, Serum, Plasma, Urine, Tissue extracts |
| Detection Limit | 1 µM |
| Standard Linear Range | 1 µM - 200 µM |
| Procedure | 1. Add samples and standards to the wells 2. Add the reaction mixture and incubate at room temperature for 30 minutes 3. Analyze using a microplate reader |
| Storage | -20°C |
For research use only, not for clinical use.
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