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| Product Name | pMD2.G Envelope Plasmid DNA (Kanamycin Resistance) |
| Catalog No. | RESK-0403 |
| Description | The pMD2.G Envelope Plasmid DNA (Kanamycin Resistance) is an envelope plasmid DNA product with kanamycin resistance, intended for AAV packaging experiments in basic research. The plasmid is supplied at a concentration of 1.0 mg/mL. Downstream purification uses a chromatography-free plasmid process, resulting in a low risk of plasmid cross-contamination, and the product is widely applicable to AAV packaging experiments in basic research. |
| Intended Use | For AAV (adeno-associated virus) packaging experiments in basic research. |
| Principle / Technology | Plasmid DNA reagent for viral packaging; purified using a chromatography-free plasmid purification process. |
| Detection Method | UV spectrophotometry Anion-exchange high-performance liquid chromatography (AEX-HPLC) Agarose gel electrophoresis (AGE) |
| Performance Range / Specifications | Concentration (A260): 1 mg/mL ±10% Purity (A260/A280): 1.8-2.0 Purity (AEX-HPLC): >85% Purity (AGE): >80% Endotoxin: <10 EU/mg |
| Reaction Conditions / Protocol | 1. Preparation Remove the vial from storage at below -65°C and thaw at room temperature for 5 min. Centrifuge briefly (3000 rpm, 30 s) to collect any liquid from the cap into the bottom of the tube. 2. Concentration verification (optional) Take 2 μL and measure the A260 value with a UV spectrophotometer; calculate the concentration and confirm that it is consistent with the labeled concentration. |
| Storage Conditions | Store upright and protected from light. -85 to -65°C: 1 year -25 to -15°C: 1 month 2 to 8°C: 1 week |
| Shelf Life | 1 year at -85 to -65°C; 1 month at -25 to -15°C; 1 week at 2 to 8°C. |
| Package Specifications | 1 mg 5 mg 10 mg |
| Product Form | Plasmid DNA solution (1 mg/mL) |
| Quality Control | Concentration (A260): 1 mg/mL ±10% Purity (A260/A280): 1.8-2.0 Purity (AEX-HPLC): >85% Purity (AGE): >80% Endotoxin: <10 EU/mg Purified using a chromatography-free plasmid purification process. |
| Purity | A260/A280: 1.8-2.0 AEX-HPLC: >85% AGE: >80% |
| Concentration | 1 mg/mL ±10% |
| Expiration Date / Stability | 1 year at -85 to -65°C; 1 month at -25 to -15°C; 1 week at 2 to 8°C. |
| Regulatory / Compliance | For research use only. |
| Application Notes / Precautions | Downstream purification uses a chromatography-free plasmid process, resulting in a low risk of plasmid cross-contamination. Thaw at room temperature for 5 min before use and centrifuge briefly (3000 rpm, 30 s) to collect the liquid at the bottom of the tube. |
For research use only, not for clinical use.
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