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| Product Name | Periodic Acid-Schiff (PAS) Staining System for Glycogen Detection |
| Catalog No. | ATRSA-0023 |
| Description | A complete histochemical staining system for detecting polysaccharides, glycogen, and carbohydrate-rich molecules in tissue sections and cell preparations. Periodic acid oxidizes vicinal diol groups in carbohydrates to aldehydes, which subsequently react with Schiff reagent to produce a magenta-colored product. The included hematoxylin counterstain provides nuclear detail for morphological context. This system is widely used in the diagnosis of glycogen storage diseases and in identifying mucin-producing adenocarcinomas. |
| Intended Use | Histochemical detection of glycogen and neutral mucopolysaccharides in tissue sections for pathological evaluation. |
| Principle / Technology | Periodic acid oxidation of 1,2-glycol groups yielding aldehydes detected by Schiff reagent |
| Detection Method | Brightfield light microscopy |
| Sample Type | Formalin-fixed paraffin-embedded tissue sections, frozen sections, cell smears |
| Performance Range / Specifications | Staining time: approximately 30 min total; magenta color intensity proportional to carbohydrate content |
| Sensitivity / LOD | Detects glycogen granules as small as 0.2 µm diameter in hepatocytes |
| Specificity | Oxidizes and detects 1,2-glycol groups in glycogen, neutral mucins, basement membranes, and fungal cell walls; diastase pretreatment provides glycogen specificity control |
| Reaction Conditions / Protocol | Deparaffinize and hydrate sections; oxidize in periodic acid 5 min; rinse; apply Schiff reagent 15 min; rinse in sulfite water; counterstain with hematoxylin 2 min; dehydrate, clear, and mount |
| Components / Formulation | Periodic acid solution (0.5%), Schiff reagent (basic fuchsin-based), hematoxylin counterstain, sulfite rinse solution |
| Storage Conditions | 2–8 °C for Schiff reagent; room temperature for other components; protect Schiff reagent from light |
| Shelf Life | 12 months for Schiff reagent; 18 months for other reagents |
| Package Specifications | 100 slide kit |
| Product Form | Liquid reagents |
| Quality Control | Each lot validated on liver tissue sections showing glycogen-rich periportal hepatocytes; diastase control confirms glycogen specificity |
| Key Features | Schiff reagent formulated for high sensitivity and low background in formalin-fixed tissue |
| Purity | Dye content as specified; filtered to remove particulates |
| Concentration | As specified; ready-to-use or concentrate format |
| Activity / Unit Definition | Staining intensity and specificity validated on reference tissue/cell preparations |
| Molecular Weight | Varies by dye component as specified in product documentation |
| Source / Origin | Synthetic dyes and biological stains; certified by Biological Stain Commission where applicable |
| pH Range / Optimal pH | pH 6.0–7.0 for Romanowsky-type stains; varies by formulation |
| Shipping Conditions | Ambient temperature; protect from light and moisture |
| Expiration Date / Stability | 24 months in unopened container; protect from light |
| Regulatory / Compliance | For in vitro diagnostic use in histology and cytology; CE-marked options available |
| Compatibility | Compatible with formalin-fixed tissue sections, blood smears, and cytological preparations |
| Recommended Buffer System | Use with provided buffer or phosphate buffer pH 6.8 as specified |
| Application Notes / Precautions | Avoid contact with skin and clothing; stains are permanent; use appropriate PPE |
| Batch-to-Batch Consistency | Staining intensity and differential contrast validated on reference specimens per lot |
For research use only, not for clinical use.
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