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| Product Name | One-Step Luciferase Assay Kit (Steady Glow) |
| Catalog No. | RESK-0153 |
| Description | This one-step firefly luciferase reporter gene assay kit features simple operation and a signal half-life of more than 5 h, with minimal luminescence change during the first 2 h, making it especially suitable for ultra-high-throughput or 384-well plate detection. No cell washing or harvesting is required before the assay; the reagent is used directly, completing cell lysis and detection in one step, eliminating reagent mixing steps and saving experimental time. The assay has a wide linear range, showing good linearity over a range of up to 200,000 cells per well in a 96-well plate. |
| Intended Use | One-step firefly luciferase reporter gene detection with a highly stable, long-half-life glow signal, particularly suited to ultra-high-throughput screening and 384-well plate formats. |
| Principle / Technology | Firefly luciferase is a protein of approximately 61 kDa. In the presence of ATP, magnesium ions, and oxygen, it catalyzes the oxidation of luciferin to oxyluciferin, emitting bioluminescence at approximately 560 nm. The single reagent formulation lyses cells and generates a steady glow-type signal with a half-life of more than 5 h. |
| Detection Method | Bioluminescence detection using a luminometer or luminescence-capable microplate reader. |
| Sample Type | Cultured cells in medium; no cell washing or collection required. |
| Performance Range / Specifications | Signal half-life of more than 5 h, with minimal luminescence change during the first 2 h. Signal values spanning up to 7 orders of magnitude, providing a large detection window. Good linearity over up to 200,000 cells per well in a 96-well plate. Signal intensity essentially unaffected after 5 freeze-thaw cycles. |
| Sensitivity / LOD | High sensitivity with a wide detection window (signal values spanning up to 7 orders of magnitude). |
| Reaction Conditions / Protocol | One-step protocol: without washing or harvesting cells, add an equal volume of detection reagent directly to the cell culture medium; cell lysis and detection are completed in one step with no reagent mixing required. Read the luminescence with a luminometer. |
| Components / Formulation | Ready-to-use single detection reagent combining cell lysis and luciferase detection components. |
| Storage Conditions | Store at -80 °C protected from light. If stored at -20 °C protected from light, use within 2 weeks. After opening, aliquot for storage (protected from light). |
| Shelf Life | 1 year for unopened reagent stored at -80 °C protected from light; use within 2 weeks when stored at -20 °C. |
| Package Specifications | 100 T 1000 T 10 x 100 T |
| Molecular Weight | Firefly luciferase: approximately 61 kDa. |
| Shipping Conditions | Shipped on dry ice. |
| Regulatory / Compliance | For research use only. Not for use in diagnostic procedures. |
| Compatibility | Particularly suitable for ultra-high-throughput or 384-well plate detection; also compatible with 96-well formats. |
| Application Notes / Precautions | No cell washing or collection is required before the assay; add the reagent directly to the culture medium. Protect the reagent from light; after opening, aliquot to avoid repeated freeze-thaw. |
For research use only, not for clinical use.
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