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| Product Name | Cell Autophagy Detection Kit (MDC Method) |
| Catalog No. | RESK-0187 |
| Description | Autophagy is a process in which cells, upon stimulation, engulf their own cytoplasm or organelles and ultimately degrade the engulfed material within lysosomes. The autophagosome is a round or oval structure enclosed by a double membrane, containing cytoplasm, long-lived proteins, abnormal protein aggregates, damaged or redundant organelles such as mitochondria, rough endoplasmic reticulum and peroxisomes, as well as viruses and bacteria. Monodansylcadaverine (MDC) is a fluorescent dye and an eosinophilic staining agent commonly used as a specific marker to detect autophagosome formation. The detection uses an excitation filter of 355-380 nm and a barrier filter of 512-530 nm. The MDC staining solution is suitable for fluorescent autophagy staining of cultured cells, can be used with FACS to determine the proportion of cells undergoing autophagy, and can be combined with EB for double staining. |
| Intended Use | Suitable for fluorescent observation of autophagy in cultured cells and for determining the proportion of cells undergoing autophagy by FACS; can be combined with EB for double staining. |
| Principle / Technology | Monodansylcadaverine (MDC) is a fluorescent dye used as a specific marker for detecting autophagosome formation. MDC staining enables fluorescent labeling of autophagosomes in cultured cells. |
| Detection Method | Fluorescence staining (excitation filter 355-380 nm, barrier filter 512-530 nm); compatible with fluorescence microscopy and flow cytometry (FACS). |
| Sample Type | Cultured cells. |
| Components / Formulation | Two-part kit (Part I and Part II) containing MDC staining solution. |
| Storage Conditions | Part I: store at -25 to -15 °C and protect from light. Part II: store at 2-8 °C. |
| Shelf Life | 1 year. |
| Package Specifications | 100 T |
| Expiration Date / Stability | Stable for 1 year when stored as recommended. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Can be combined with EB for double staining; compatible with FACS analysis. |
For research use only, not for clinical use.
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