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| Product Name | Firefly Glo Luciferase Reporter Gene Assay Kit |
| Catalog No. | RESK-0138 |
| Description | A glow-type firefly luciferase reporter gene assay kit featuring high sensitivity and a stable luminescent signal, suitable for high-throughput detection of luciferase expression in mammalian cells. The kit employs a simple add-mix-read workflow that requires no autoinjector and no medium removal or centrifugation steps, greatly simplifying the experimental procedure, and has no obvious pungent odor. It is compatible with a variety of commonly used cell culture media, including RPMI 1640, DMEM, MEM-alpha, F12, and DMEM/F12, with a signal half-life of approximately 2 h at 22°C in all of them, meeting the needs of most high-throughput assays. |
| Intended Use | High-throughput detection of firefly luciferase reporter gene expression in mammalian cells. For research use only. |
| Principle / Technology | Firefly luciferase, a protein of approximately 61 kDa, catalyzes the oxidation of luciferin to oxyluciferin in the presence of ATP, magnesium ions, and oxygen, emitting bioluminescence at a wavelength of approximately 560 nm that can be measured with a luminometer. This glow-type formulation provides a stable luminescent signal (half-life approximately 2 h), allowing greater flexibility in experimental design compared with flash-type assays. |
| Detection Method | Bioluminescence (glow-type); measure luminescence at approximately 560 nm using a luminometer or a microplate reader equipped with a luminescence module (full-wavelength detection over 350-700 nm). |
| Sample Type | Cultured mammalian cells expressing firefly luciferase |
| Performance Range / Specifications | Stable signal: long half-life of approximately 2 h (at 22°C), ideal for high-throughput detection. Simple operation: only add the reagent and read the signal to complete the assay. Broad compatibility: suitable for a variety of commonly used cell culture media, such as RPMI 1640, DMEM, MEM-alpha, F12, and DMEM/F12. |
| Sensitivity / LOD | High sensitivity |
| Reaction Conditions / Protocol | Add an equal volume of the assay reagent to the cell culture, mix, and measure the luminescent signal (add-mix-read). No autoinjector is required, and there is no need to remove the culture medium or centrifuge. Signal half-life is approximately 2 h at 22°C. |
| Components / Formulation | Glow-type firefly luciferase substrate and assay buffer (lyophilized substrate to be reconstituted before use) |
| Storage Conditions | Store the unopened kit at -20°C. After reconstitution and aliquoting, store the glow-type firefly luciferase substrate protected from light at -70°C for up to 1 year, or at -20°C for no more than 1 month (short term). |
| Shelf Life | 1 year for the unopened kit stored at -20°C |
| Package Specifications | 100 T 1000 T |
| Product Form | Kit (lyophilized substrate with assay buffer) |
| Molecular Weight | Firefly luciferase: approximately 61 kDa |
| Shipping Conditions | Shipped on dry ice |
| Expiration Date / Stability | Unopened kit: 1 year at -20°C. Reconstituted and aliquoted substrate: 1 year at -70°C protected from light, or no more than 1 month at -20°C. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Compatible with commonly used cell culture media, including RPMI 1640, DMEM, MEM-alpha, F12, and DMEM/F12; signal half-life is approximately 2 h (at 22°C) in all of these media. |
| Application Notes / Precautions | A fluorescence microscope cannot be used for luciferase detection; an instrument with a bioluminescence module is required (full-wavelength 350-700 nm detection), such as a single-tube luminometer or a multi-mode microplate reader. After cell lysis, lysates can be stored at -80°C for up to 6 months or at -20°C for up to 1 month, similar to general protein storage. If the luminescence signal is too high, dilute the lysed sample or shorten the cell incubation time after transfection. |
For research use only, not for clinical use.
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