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| Product Name | Cell Total Iron Assay Kit (Colorimetric) |
| Catalog No. | RESK-0183 |
| Description | Iron is one of the essential elements for the human body and plays an important role in metabolism and overall health. Iron deficiency in the blood can lead to iron-deficiency anemia. Iron is present in all cells of the human body, and it is found in various tissues and organs, including the endocrine glands. The liver, spleen, and lung tissues contain relatively abundant iron. An adult human body contains about 3-5 g of iron, most of which exists in the form of protein complexes, with only a very small fraction present as free ions. |
| Intended Use | For quantitative determination of total iron content in cell samples. |
| Principle / Technology | This kit quantifies cellular iron by a colorimetric method. After cell lysis, sodium sulfite reduces Fe3+ in the sample to Fe2+. Fe2+ then forms a blue complex with the chromogenic reagent under acidic conditions, with an absorption peak at 593 nm. The total iron content is calculated by measuring the absorbance at this wavelength. |
| Detection Method | Colorimetric assay; absorbance measured at 593 nm. |
| Sample Type | Cell samples (cell lysates). |
| Reaction Conditions / Protocol | Cells are lysed prior to the assay; the chromogenic reaction is performed under acidic conditions. |
| Storage Conditions | Store at 2-8 °C and protect from light. |
| Shelf Life | 12 months. |
| Package Specifications | 48 T / 96 T |
| Expiration Date / Stability | Stable for 12 months when stored at 2-8 °C protected from light. |
| Regulatory / Compliance | For research use only. |
For research use only, not for clinical use.
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