| Product Name | NAD/NADH Assay Reagent |
| Catalog No. | CMTR-HMM-0017 |
| Description | NAD+ is a chemical substance in the human body, a coenzyme present in all living cells. It consists of two nucleotides connected by a phosphate group, with one nucleotide containing an adenine base and the other nicotinamide. It serves as an energy source for hundreds of biochemical processes in the human body, participating in maintaining mitochondrial function, DNA repair, antioxidant activities, and various physiological processes. NADH is a coenzyme present in all living cells; it consists of two nucleotides connected by a 5'-phosphate group, one of which contains an adenine base and the other a nicotinamide base. It serves both as a basic metabolite and as a cofactor. |
| Application | This product can quantify NAD+ and NADH in mammalian and other species samples and measure their ratio. |
| Principle | Both NADt (total NAD+ and NADH) and NADH levels can be easily measured; the NAD+ level can be easily calculated by subtracting NADH from NADt. The measurement results are read at 450 nm. |
| Assay Type | Quantitative |
| Detection Method | Colorimetric |
| Applicable Instruments | Microplate reader |
| Detection Time | 2 h |
| Sample Type | Cell lysate, Serum, Tissue lysate, Urine |
| Standard Linear Range | 400 nM - 2000 nM |
| Procedure | 1. Extract samples from cells/tissues using extraction buffer and remove proteins using a spin column 2. For NADH measurement, heat the sample to 60°C for 30 minutes to decompose NAD+, then cool on ice (this step is not required when measuring total NAD+/NADH) 3. Add the sample and standard to the wells. 4. Add the reaction mixture and incubate at room temperature for 5 minutes to convert NAD to NADH. 5. Add the NADH chromogen and incubate for 1–4 hours while performing reaction cycles. 6. Analyze multiple times during the 1–4 hour incubation period using an enzyme-linked immunosorbent assay (ELISA) reader. 7. The reaction can be stopped using a stop solution. |
| Storage | -20°C |
For research use only, not for clinical use.
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