| Product Name | Mouse Phospholipase A2, membrane associated (PLA2G2A) ELISA Kit |
| Catalog No. | PAK-HMM-0036 |
| Description | Group IIA phospholipase A2 (platelets, synovial fluid) (PLA2G2A), also known as PLA2 or sPLA2, is an enzyme encoded by the PLA2G2A gene. It is a member of the phospholipase A2 family (PLA2), which constitutes a diverse family of enzymes in terms of sequence, function, localization, and divalent cation requirements. The gene product belongs to group II, which contains the secreted form of PLA2, an extracellular enzyme with low molecular weight that requires calcium ions for catalysis. |
| Test Species | Mouse |
| Synonyms | PLA2; MOM1; PLA2B; PLA2L; PLA2S; PLAS1; sPLA2(platelets,synovial fluid); Non-pancreatic secretory phospholipase A2; Phosphatidylcholine 2-acylhydrolase 2A；Phospholipase A2, Group IIA |
| Applications | The kit detects PLA2G2A in mice in samples without significant cross-reactivity with its analogs. |
| Sample Type | Serum, plasma, tissue homogenates and other biological fluids. |
| Detection Methods | Sandwich ELISA |
| Sensitivity | 8 pg/mL |
| Detection Range | 15.6-1000 pg/mL |
| Accuracy | The intraplate coefficient of variation is less than 10% and the interplate coefficient of variation is less than 10%. |
| Storage | 2-8°C |
| Shelf Life | 6 months |
| Detection Principle | The kit is based on double antibody sandwich enzyme-linked immunosorbent assay (ELISA). To the microtiter wells pre-coated with mouse group IIA phospholipase A2 (platelets, synovial fluid) (PLA2G2A) capture antibody, add the sample, standard, biotin-labeled detection antibody, HRP enzyme conjugate sequentially, and then incubate and wash, and then use the substrate TMB to develop the color, which will be converted into blue color catalyzed by HRP, and then converted into yellow color under the effect of acid. The final color is yellow. There is a positive correlation between the color and mouse group IIA phospholipase A2 (platelets, synovial fluid) (PLA2G2A) in the sample. The absorbance (OD value) is measured at 450nm with an enzyme marker to calculate the sample concentration. |
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