| Product Name | Human Thrombospondin 2 (THBS2) ELISA Kit |
| Catalog No. | PAK-HMM-0004 |
| Description | Platelet Reactive Protein-2 is a protein encoded by the THBS2 gene. The protein encoded by this gene belongs to the platelet response protein family. It is disulfide-linked homotrimeric glycoprotein which mediates cell-cell and cell-matrix interactions. Its role in cancer is controversial and comparative research has shown both positive and negative effects on cancer development. Diseases associated with THBS2 include disc disease and back pain. Its related pathways include glycosylation disorders and miRNA targets in the ECM and membrane receptors. |
| Test Species | Human |
| Synonyms | TSP2 |
| Applications | The kit detects human THBS2 in samples without significant cross-reactivity with its analogs. |
| Sample Type | Plasma |
| Detection Methods | Sandwich ELISA |
| Sensitivity | 0.65 ng/mL |
| Detection Range | 1.56-100 ng/mL |
| Accuracy | The intraplate coefficient of variation is less than 10% and the interplate coefficient of variation is less than 10%. |
| Storage | 2-8°C |
| Shelf Life | 6 months |
| Detection Principle | The kit is based on enzyme-linked immunosorbent assay (ELISA) using double antibody sandwich method. To the microtiter wells pre-coated with human platelet reactive protein 2 (THBS2) capture antibody, the sample, standard, biotin-labeled detection antibody, HRP enzyme conjugate are added sequentially, with warming and washing, and then the color is developed with the substrate TMB, which is converted to blue color catalyzed by HRP, and then converted to the final yellow color under the action of acid. The shade of color is positively correlated with human platelet reactive protein 2 (THBS2) in the sample. The absorbance (OD value) is measured at 450nm wavelength with an enzyme marker to calculate the sample concentration. |
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