Cat.No: HTK-HMM-0024 Datasheet
Specification Quantities
| Product Name | Mouse Lysophosphatidylcholine Acyltransferase 4 (LPCAT4) Multiplex Assay Kit-FLIA |
| Catalog No. | HTK-HMM-0024 |
| Description | Lysophosphatidylcholine acyltransferase 4 (LPCAT4) is mainly involved in phospholipid metabolism, especially playing a key role in the synthesis of phosphatidylcholine. It is capable of converting lysophosphatidylcholine to phosphatidylcholine, a process that is essential for maintaining cell membrane stability and function. |
| Test Species | Mouse |
| Synonyms | AYTL3; AGPAT7; LPAAT; LPEAT2; Lysophosphatidylethanolamine Acyltransferase 2; Acyltransferase Like 3; 1-Acylglycerol-3-Phosphate O-Acyltransferase 7; Plasmalogen synthase |
| Applications | This kit is used for the detection of Lysophosphatidyltransferase 4 (LPCAT4) and other multi-factors, and there is no significant cross-reactivity with other similar substances. No more than 8 indicators of multi-factors can be mixed in a single assay. |
| Sample Type | Tissue homogenates and other biological fluids. |
| Detection Methods | FLIA |
| Estimated Measurement Time | 3.5 h |
| Detection Range | 0.04-40 ng/mL |
| Sensitivity | 0.013 ng/mL |
| Accuracy | Intra-lot variation: CV<10%; Inter-lot variation: CV<12%. |
| Detection Principle | The antibodies of the multi-factor detection kit (flow fluorescence luminescence method) such as lysolytic lecithin acyltransferase 4(LPCAT4), are coated on magnetic beads to prepare solid-phase carriers. Standards or specimens and magnetic beads are added to the microwells, respectively. Among them, the hemolytic lecithin acyltransferase 4(LPCAT4) and other multi-factor detection kits (flow cytometry fluorescence luminescence method) are combined with the antibodies attached to the solid-phase carrier. Then, the biotinylated hemolytic lecithin acyltransferase 4(LPCAT4) and other multi-factor detection kits (flow cytometry fluorescence luminescence method) antibodies are added. After washing off the unbound biotinylated antibodies, add the PE-labeled avidin, and after thorough washing again, it can be used for reading on the machine. The MFI value is positively correlated with the multi-factor detection kits such as lysolytic lecithin acyltransferase 4(LPCAT4) in the samples (flow cytometry fluorescence luminescence method). |
| Procedures | 1. Prepare standards, reagents, and samples before the experiment; 2. Add samples (standards, samples, magnetic beads) 100 μL of standards or samples and 10 μL of magnetic beads, and incubate for 90 minutes at 37°C on an enzyme plate oscillator; 3. Drain the plate magnetically, add 100 μL of Detection Solution A, and incubate at 37°C for 60 minutes; 4. Wash the plate 3 times with magnetic suction; 5. Add 100 μL of Detection Solution B and incubate at 37°C for 30 minutes with shaking; 6. Wash the plate 3 times with magnetic suction; 7. Add 100μL of Sheath Solution, vortex for 2 minutes, and read. |
|
There is no product in your cart. |
Copyright © 2025 Alta DiagenoTech. All rights reserved.
