Cat.No: HTK-HMM-0008 Datasheet
Specification Quantities
| Product Name | Human Lysophosphatidylglycerol Acyltransferase 1 (LPGAT1) Multiplex Assay Kit-FLIA |
| Catalog No. | HTK-HMM-0008 |
| Description | LPGAT1 encodes a protein belonging to the lysophospholipid acyltransferase family, whose function involves cell membrane remodeling and cardiolipin synthesis, which is essential for maintaining cell membrane stability and function. |
| Test Species | Human |
| Synonyms | FAM34A1; NET8; Family With Sequence Similarity 34, Member A |
| Applications | This kit is used for the detection of lysophosphatidylglycerol acyltransferase 1 (LPGAT1) and other multi-factors, and there is no significant cross-reactivity with other similar substances after testing. No more than 8 indicators of multi-factors can be mixed in a single test. |
| Sample Type | Tissue homogenates, cell lysates and other biological fluids. |
| Detection Methods | FLIA |
| Estimated Measurement Time | 3.5 h |
| Detection Range | 0.01-10 ng/mL |
| Sensitivity | 0.003 ng/mL |
| Accuracy | Intra-lot variation: CV<10%; Inter-lot variation: CV<12%. |
| Detection Principle | The antibodies of the multi-factor detection kit (flow fluorescence luminescence method), such as lysolytic phosphatidylglycerol acyltransferase 1(LPGAT1), are coated on magnetic beads to prepare solid-phase carriers. Standards or specimens and magnetic beads are added to the microwells, respectively. Among them, the multi-factor detection kits, such as lysophosphatidylglycerol acyltransferase 1(LPGAT1) (flow cytometry fluorescence luminescence method) are combined with the antibodies linked to the solid-phase carrier, and then biotinylated antibodies such as lysophosphatidylglycerol acyltransferase 1(LPGAT1) and other multi-factor detection kits (flow cytometry fluorescence luminescence method) are added. After washing off the unbound biotinylated antibodies, add PE-labeled avidin, and thoroughly wash again before taking the readings on the machine. The MFI value is positively correlated with the multi-factor detection kits such as lysophosphatidylglycerol acyltransferase 1(LPGAT1) in the samples (flow cytometry fluorescence spectrometry). |
| Procedures | 1. Prepare standards, reagents, and samples before the experiment; 2. Add samples (standards, samples, magnetic beads) 100 μL of standards or samples and 10 μL of magnetic beads, and incubate for 90 minutes at 37°C on an enzyme plate oscillator; 3. Drain the plate magnetically, add 100 μL of Detection Solution A, and incubate at 37°C for 60 minutes; 4. Wash the plate 3 times with magnetic suction; 5. Add 100 μL of Detection Solution B and incubate at 37°C for 30 minutes with shaking; 6. Wash the plate 3 times with magnetic suction; 7. Add 100μL of Sheath Solution, vortex for 2 minutes, and read. |
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