Cat.No: HTK-HMM-0006 Datasheet
Specification Quantities
| Product Name | Mouse Lysophosphatidic Acid Receptor 3 (LPAR3) Multiplex Assay Kit-FLIA |
| Catalog No. | HTK-HMM-0006 |
| Description | The primary intracellular role of lysophosphatidic acid receptor 3 (LPAR3) is to trigger intracellular signaling through members of the G-protein-coupled receptor family, thereby affecting biological behaviors such as cell proliferation, survival, infiltration, and metastasis. |
| Test Species | Mouse |
| Synonyms | GPCR; EDG7; HOFNH30; LP-A3; LPA3; LPAR3; Endothelial Differentiation, Lysophosphatidic Acid G-Protein-Coupled Receptor 7; Lysophosphatidic acid receptor Edg-7 |
| Applications | This kit is used for the detection of lysophosphatidic acid receptor 3 (LPAR3) and other multi-factors, tested and other similar substances without significant cross-reactivity. No more than 8 indicators of multi-factors can be mixed in a single test. |
| Sample Type | Tissue homogenates and other biological fluids. |
| Detection Methods | FLIA |
| Estimated Measurement Time | 3.5 h |
| Detection Range | 0.01-10 ng/mL |
| Sensitivity | 0.003 ng/mL |
| Accuracy | Intra-lot variation: CV<10%; Inter-lot variation: CV<12%. |
| Detection Principle | The antibodies of multi-factor detection kits, such as lysophosphatidic acid receptor 3(LPAR3) (flow fluorescence luminescence method) are coated on magnetic beads to make solid-phase carriers. Standard substances or specimens and magnetic beads are added to the microwells, respectively. The antibodies of multi-factor detection kits, such as lysophosphatidic acid receptor 3(LPAR3) (flow fluorescence luminescence method) are bound to the solid-phase carriers. Then add biotinylated lysophosphatidic acid receptor 3(LPAR3) and other multi-factor detection kit antibodies (flow cytometry fluorescence luminescence method). After washing off the unbound biotinylated antibodies, add PE-labeled avidin, and thoroughly wash again before reading the data on the machine. The MFI value is positively correlated with the multi-factor detection kits such as lysophosphatidic acid receptor 3(LPAR3) in the samples (flow fluorescence luminescence method). |
| Procedures | 1. Prepare standards, reagents, and samples before the experiment; 2. Add samples (standards, samples, magnetic beads) 100 μL of standards or samples and 10 μL of magnetic beads, and incubate for 90 minutes at 37°C on an enzyme plate oscillator; 3. Drain the plate magnetically, add 100 μL of Detection Solution A, and incubate at 37°C for 60 minutes; 4. Wash the plate 3 times with magnetic suction; 5. Add 100 μL of Detection Solution B and incubate at 37°C for 30 minutes with shaking; 6. Wash the plate 3 times with magnetic suction; 7. Add 100μL of Sheath Solution, vortex for 2 minutes, and read. |
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