Cat.No: HTK-HMM-0027 Datasheet
Specification Quantities
| Product Name | General Lysophosphatidylcholine (LPC) ELISA Kit |
| Catalog No. | HTK-HMM-0027 |
| Description | Lysophosphatidylcholine (LPC) plays an important role in cell membrane composition, signaling, and cellular metabolic processes. It is hydrophilic and lipophilic and is an important emulsifier that can affect the fluidity and stability of cell membranes. LPC is a metabolite of phosphatidylcholine (PC), which participates in the construction of cell membranes and signaling processes, ensures the fluidity and integrity of cell membranes, regulates intercellular signaling, and maintains normal metabolic functions of cells. |
| Test Species | General |
| Synonyms | lysoPC; Lysolecithin; 1-Palmitoyl-sn-Glycero-3-Phosphocholine |
| Applications | This kit is used for the detection of Lysophosphatidylcholine (LPC), there is no significant cross-reactivity with other similar substances. |
| Sample Type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
| Detection Methods | Competitive ELISA |
| Estimated Measurement Time | 2 h |
| Detection Range | 78.125-20,000 ng/mL |
| Sensitivity | 27.142 ng/mL |
| Accuracy | Intra-lot variation: CV<10%; Inter-lot variation: CV<12%. |
| Detection Principle | This kit uses competitive inhibition enzyme-linked immunosorbent assay to determine the level of the substance to be tested in the specimen. The microplate is coated with hemolytic phosphatidylcholine (LPC) monoclonal antibody to form a solid-phase carrier. At the same time, biotin-labeled antigen and the antigen to be tested (standard or sample) are added to the microplate Wells coated with the antibody. The antigen to be tested and the biotin-labeled antigen competed for binding to the specific antibody. After incubation, the unbound substances are washed off. Then, HRP-labeled avidin is added. After incubation and thorough washing, the substrate TMB is added for color development. TMB is converted into blue under the catalysis of peroxidase and then into the final yellow under the action of acid. The higher the concentration of the specimen to be tested is, the more inhibited the binding of the labeled antigen and antibody will be, and the lighter the color will be. The depth of color development is positively correlated with the amount of enzyme, but negatively correlated with the content of the substance to be tested in the sample. The absorbance (O.D. value) is measured at a wavelength of 450nm using an microplate reader, and the sample concentration is calculated. |
| Procedures | 1. Prepare standards, reagents, and samples before the experiment; 2. Add 50 µL of sample (standard and sample), add 50 µL of Detection Solution A (prepared before use); incubate at 37°C for 1 hour. 3. Wash the plate 3 times; 4. Add 100 µL of Detection Solution B; incubate at 37°C for 30 min; 5. Wash the plate 5 times; 6. Add TMB Substrate 90 µL and incubate at 37°C for 10-20 minutes. 7. Add 50 µL of Termination Solution and read immediately at 450 nm. |
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