Cat.No: HTK-HMM-0007 Datasheet
Specification Quantities
| Product Name | Human Lysophosphatidylglycerol Acyltransferase 1 (LPGAT1) ELISA Kit |
| Catalog No. | HTK-HMM-0007 |
| Description | LPGAT1 encodes a protein belonging to the lysophospholipid acyltransferase family, whose function involves cell membrane remodeling and cardiolipin synthesis, which is essential for maintaining cell membrane stability and function. |
| Test Species | Human |
| Synonyms | FAM34A1; NET8; Family With Sequence Similarity 34, Member A |
| Applications | This kit is used for the detection of Lysophosphatidylglycerol acyltransferase 1 (LPGAT1), and there is no significant cross-reactivity with other similar substances. |
| Sample Type | Tissue homogenates, cell lysates and other biological fluids. |
| Detection Methods | Sandwich ELISA |
| Estimated Measurement Time | 3 h |
| Detection Range | 0.156-10 ng/mL |
| Sensitivity | 0.059 ng/mL |
| Accuracy | Intra-lot variation: CV<10%; Inter-lot variation: CV<12%. |
| Detection Principle | The hemolytic phosphatidylglycerol acyltransferase 1(LPGAT1) antibody is coated in a 96-well microplate to form a solid-phase carrier. Standards or specimens are added to the microplates, respectively, and the hemolytic phosphatidylglycerol acyltransferase 1(LPGAT1) is bound to the antibody linked to the solid-phase carrier. Then, biotinylated lysophosphatidylglycerol acyltransferase 1(LPGAT1) antibody is added. After washing off the unbound biotinylated antibody, HRP-labeled avidin is added. After thorough washing again, TMB substrate is added for color development. TMB is converted into blue under the catalysis of peroxidase and then into the final yellow under the action of acid. The depth of the color is positively correlated with the lysophosphatidylglycerol acyltransferase 1(LPGAT1) in the sample. The absorbance (O.D. value) is measured at a wavelength of 450nm using an microplate reader, and the sample concentration is calculated. |
| Procedures | 1. Preparation of standards, reagents, and samples before the experiment; 2. Add 100 µL of sample (standard and sample) and incubate at 37°C for 1 hour; 3. Aspirate, add Detection Solution A 100 µL, incubate at 37°C for 1 hour; 4. Wash the plate 3 times; 5. Add 100 µL of Detection Solution B and incubate at 37°C for 30 minutes. 6. Wash the plate 5 times; 7. Add TMB Substrate 90 µL, incubate at 37°C for 10-20 minutes. 8. Add 50 µL of Termination Solution and read at 450 nm immediately. |
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