Cat.No: HTK-HMM-0022 Datasheet
Specification Quantities
| Product Name | Mouse Lysophosphatidylcholine Acyltransferase 4 (LPCAT4) ELISA Kit |
| Catalog No. | HTK-HMM-0022 |
| Description | Lysophosphatidylcholine acyltransferase 4 (LPCAT4) is mainly involved in phospholipid metabolism, especially playing a key role in the synthesis of phosphatidylcholine. It is capable of converting lysophosphatidylcholine to phosphatidylcholine, a process that is essential for maintaining cell membrane stability and function. |
| Test Species | Mouse |
| Synonyms | AYTL3; AGPAT7; LPAAT; LPEAT2; Lysophosphatidylethanolamine Acyltransferase 2; Acyltransferase Like 3; 1-Acylglycerol-3-Phosphate O-Acyltransferase 7; Plasmalogen synthase |
| Applications | This kit is used for the detection of Lysophosphatidyltransferase 4 (LPCAT4), there is no significant cross-reactivity with other similar substances. |
| Sample Type | Tissue homogenates and other biological fluids. |
| Detection Methods | Sandwich ELISA |
| Estimated Measurement Time | 3 h |
| Detection Range | 0.625-40 ng/mL |
| Sensitivity | 0.212 ng/mL |
| Accuracy | Intra-lot variation: CV<10%; Inter-lot variation: CV<12%. |
| Detection Principle | The hemolytic lecithin acyltransferase 4(LPCAT4) antibody is coated in a 96-well microplate to form a solid-phase carrier. Standards or specimens are added to the microplates, respectively, and the hemolytic lecithin acyltransferase 4(LPCAT4) is bound to the antibody linked to the solid-phase carrier. Then, add the biotinylated lysolytic lecithin acyltransferase 4(LPCAT4) antibody. After washing off the unbound biotinylated antibody, add HRP-labeled avidin, thoroughly wash again, and then add TMB substrate for color development. TMB is converted into blue under the catalysis of peroxidase and then into the final yellow under the action of acid. The depth of color is positively correlated with the hemolytic lecithin acyltransferase 4(LPCAT4) in the sample. The absorbance (O.D. value) is measured at a wavelength of 450nm using an microplate reader, and the sample concentration is calculated. |
| Procedures | 1. Preparation of standards, reagents, and samples before the experiment; 2. Add 100 µL of sample (standard and sample) and incubate at 37°C for 1 hour; 3. Aspirate, add Detection Solution A 100 µL, incubate at 37°C for 1 hour; 4. Wash the plate 3 times; 5. Add 100 µL of Detection Solution B and incubate at 37°C for 30 minutes. 6. Wash the plate 5 times; 7. Add TMB Substrate 90 µL, incubate at 37°C for 10-20 minutes. 8. Add 50 µL of Termination Solution and read at 450 nm immediately. |
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