Cat.No: HTK-HMM-0013 Datasheet
Specification Quantities
| Product Name | Mouse Lysophosphatidylcholine Acyltransferase 1 (LPCAT1) Multiplex Assay Kit-FLIA |
| Catalog No. | HTK-HMM-0013 |
| Description | The main role of lysophosphatidyl transferase 1 (LPCAT1) is to participate in cell membrane remodeling and signal transduction processes by catalyzing the conversion of lysophosphatidylcholine (LPC) to phosphatidylcholine (PC). LPCAT1 plays a key role in cell membrane remodeling by reacylating LPC to PC through the Lands cycle pathway, thereby altering the phospholipid composition of the cell membrane and affecting the structure and function of the cell membrane. |
| Test Species | Mouse |
| Synonyms | AYTL2; PFAAP3; Acyltransferase Like 2; 1-alkylglycerophosphocholine O-acetyltransferase; Phosphonoformate immuno-associated protein 3; Acetyl-CoA:lyso-PAF acetyltransferase |
| Applications | This kit is used for the detection of hemolytic lecithin phosphatidyltransferase 1 (LPCAT1) and other multi-factors, tested and other similar substances without significant cross-reactivity. No more than 8 indicators of multi-factors can be mixed in a single test. |
| Sample Type | Serum, plasma, tissue homogenates and other biological fluids. |
| Detection Methods | FLIA |
| Estimated Measurement Time | 3.5 h |
| Detection Range | 0.01-10 ng/mL |
| Sensitivity | 0.003 ng/mL |
| Accuracy | Intra-lot variation: CV<10%; Inter-lot variation: CV<12%. |
| Detection Principle | The antibodies of the multi-factor detection kit (flow fluorescence luminescence method) such as lysolytic lecithin acyltransferase 1(LPCAT1), are coated on magnetic beads to prepare solid-phase carriers. Standards or specimens and magnetic beads are added to the microwells, respectively. Among them, the hemolytic lecithin acyltransferase 1(LPCAT1) and other multi-factor detection kits (flow cytometry fluorescence luminescence method) are combined with the antibodies attached to the solid-phase carrier. Then, the biotinylated hemolytic lecithin acyltransferase 1(LPCAT1) and other multi-factor detection kits (flow cytometry fluorescence luminescence method) antibodies are added. After washing off the unbound biotinylated antibodies, add the PE-labeled avidin, and after thorough washing again, it can be used for reading on the machine. The MFI value is positively correlated with the multi-factor detection kits such as lysolytic lecithin acyltransferase 1(LPCAT1) in the samples (flow cytometry fluorescence luminescence method). |
| Procedures | 1. Prepare standards, reagents, and samples before the experiment; 2. Add samples (standards, samples, magnetic beads) 100 μL of standards or samples and 10 μL of magnetic beads, and incubate for 90 minutes at 37°C on an enzyme plate oscillator; 3. Drain the plate magnetically, add 100 μL of Detection Solution A, and incubate at 37°C for 60 minutes; 4. Wash the plate 3 times with magnetic suction; 5. Add 100 μL of Detection Solution B and incubate at 37°C for 30 minutes with shaking; 6. Wash the plate 3 times with magnetic suction; 7. Add 100μL of Sheath Solution, vortex for 2 minutes, and read. |
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