Cat.No: HTK-HMM-0002 Datasheet
Specification Quantities
| Product Name | Human Lysophosphatidic Acid Receptor 1 (LPAR1) Multiplex Assay Kit-FLIA |
| Catalog No. | HTK-HMM-0002 |
| Description | LPAR1 (lysophosphatidic acid receptor 1) is an important G protein-coupled receptor in the human body, which plays an important role in physiological processes such as cell signaling, cell proliferation, cell migration, and cell adhesion. During these physiological processes, LPAR1 can bind to lysosomal acid-like deoxygenated neuronal activation precursor (LPA) produced by lysosomes and mediate cellular signaling. Activation of LPAR1 leads to an increase in intracellular calcium concentration, which in turn activates a variety of signaling pathways and affects cellular function. |
| Test Species | Human |
| Synonyms | EDG2; Gpcr26; LPA1; Mrec1.3; edg-2; rec.1.3; vzg-1; Endothelial Differentiation, Lysophosphatidic Acid G-Protein-Coupled Receptor 2 |
| Applications | This kit is used for the detection of lysophosphatidic acid receptor 1 (LPAR1) and other multi-factors, tested and other similar substances without significant cross-reactivity. No more than 8 indicators of multi-factors can be mixed in a single test. |
| Sample Type | Tissue homogenates, cell lysates and other biological fluids. |
| Detection Methods | FLIA |
| Estimated Measurement Time | 3.5 h |
| Detection Range | 0.02-20 ng/mL |
| Sensitivity | 0.007 ng/mL |
| Accuracy | Intra-lot variation: CV<10%; Inter-lot variation: CV<12%. |
| Detection Principle | The antibodies of multi-factor detection kits, such as lysophosphatidic acid receptor 1(LPAR1) (flow cytometry, fluorescence luminescence method), are coated on magnetic beads to make solid-phase carriers. Standard substances or specimens and magnetic beads are added to the microwells, respectively. The antibodies of multi-factor detection kits, such as lysophosphatidic acid receptor 1(LPAR1) (flow cytometry fluorescence luminescence method) are bound to the solid-phase carriers. Then add biotinylated lysophosphatidic acid receptor 1(LPAR1) and other multi-factor detection kit antibodies (flow cytometry fluorescence luminescence method). After washing off the unbound biotinylated antibodies, add PE-labeled avidin, and thoroughly wash again before taking readings on the machine. The MFI value is positively correlated with the multi-factor detection kits such as lysophosphatidic acid receptor 1(LPAR1) in the samples (flow fluorescence luminescence method). |
| Procedures | 1. Prepare standards, reagents, and samples before the experiment; 2. Add samples (standards, samples, magnetic beads) 100 μL of standards or samples and 10 μL of magnetic beads, and incubate for 90 minutes at 37°C on an enzyme plate oscillator; 3. Drain the plate magnetically, add 100 μL of Detection Solution A, and incubate at 37°C for 60 minutes; 4. Wash the plate 3 times with magnetic suction; 5. Add 100 μL of Detection Solution B and incubate at 37°C for 30 minutes with shaking; 6. Wash the plate 3 times with magnetic suction; 7. Add 100μL of Sheath Solution, vortex for 2 minutes, and read. |
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