Cat.No: HTK-HMM-0028 Datasheet
Specification Quantities
| Product Name | General Lysophosphatidylcholine (LPC) |
| Catalog No. | HTK-HMM-0028 |
| Description | Lysophosphatidylcholine (LPC) plays an important role in cell membrane composition, signaling, and cellular metabolic processes. It is hydrophilic and lipophilic and is an important emulsifier that can affect the fluidity and stability of cell membranes. LPC is a metabolite of phosphatidylcholine (PC), which participates in the construction of cell membranes and signaling processes, ensures the fluidity and integrity of cell membranes, regulates intercellular signaling, and maintains normal metabolic functions of cells. |
| Test Species | General |
| Synonyms | lysoPC; Lysolecithin; 1-Palmitoyl-sn-Glycero-3-Phosphocholine |
| Applications | This kit is used for the detection of lysophosphatidylcholine (LPC) and other multi-factors, after testing and other similar substances without significant cross-reactivity. No more than 8 indicators of multi-factors can be mixed in a single test. |
| Sample Type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
| Detection Methods | FLIA |
| Estimated Measurement Time | 1.5 h |
| Detection Range | 19.53-20000 ng/mL |
| Sensitivity | 6.51 ng/mL |
| Accuracy | Intra-lot variation: CV<10%; Inter-lot variation: CV<12%. |
| Detection Principle | The antibodies of the multi-factor detection kit (flow fluorescence luminescence method) such as lysolytic phosphatidylcholine (LPC), are coated on magnetic beads to prepare solid-phase carriers. Standard substances or specimens, magnetic beads, and labeled VEGFA are added to the microwells, respectively. Labeled lysophosphatidylcholine (LPC) and other multi-factor detection kits (flow cytometry fluorescence luminescence method) compete for binding with antibodies bound to solid-phase carriers. Then, after washing off the unbound substances, PE-labeled avidin is added. After thorough washing again, you can take the reading on the machine. The MFI value is negatively correlated with the multi-factor detection kits (flow fluorescence fluorescence method) such as lysophosphatidylcholine (LPC) in the samples. |
| Procedures | 1. Standard, reagent, and sample preparation before experiment; 2. Add (standard, sample, magnetic beads, assay solution A) 50 μL of standard or sample and 10 μL of magnetic beads, add Detection Solution A 50 μL, incubate for 60 minutes at 37°C on a plate shaker; 3. Wash the plate with magnetic suction 3 times; 4. Add 100 μL of Detection Solution B and incubate at 37°C for 30 minutes with shaking; 5. Wash the plate magnetically 3 times; 6. Add 100 μL of Sheath Solution, vortex for 2 minutes and read. |
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