Cat.No: HTK-HMM-0018 Datasheet
Specification Quantities
| Product Name | Human Lysophosphatidylcholine Acyltransferase 3 (LPCAT3) ELISA Kit |
| Catalog No. | HTK-HMM-0018 |
| Description | Lysophosphatidyl transferase 3 (LPCAT3) has a variety of important roles in organisms, mainly including participation in lipid metabolism and regulation of cell death processes. |
| Test Species | Human |
| Synonyms | MBOAT5; C3F; OACT5; Nessy; LPSAT; Membrane Bound O-AcylTransferase Domain Containing 5; Lysophospholipid Acyltransferase 5; Lysophosphatidylserine acyltransferase |
| Applications | This kit is used for the detection of Lysophosphatidyltransferase 3 (LPCAT3), there is no significant cross-reactivity with other similar substances. |
| Sample Type | Serum, plasma, tissue homogenates and other biological fluids. |
| Detection Methods | Sandwich ELISA |
| Estimated Measurement Time | 3 h |
| Detection Range | 0.625-40 ng/mL |
| Sensitivity | 0.221 ng/mL |
| Accuracy | Intra-lot variation: CV<10%; Inter-lot variation: CV<12%. |
| Detection Principle | The hemolytic lecithin acyltransferase 3(LPCAT3) antibody is coated in a 96-well microplate to form a solid-phase carrier. Standards or specimens are added to the microplates, respectively, and the hemolytic lecithin acyltransferase 3(LPCAT3) is bound to the antibody linked to the solid-phase carrier. Then, add the biotinylated lysolytic lecithin acyltransferase 3(LPCAT3) antibody. After washing off the unbound biotinylated antibody, add HRP-labeled avidin, thoroughly wash again, and then add TMB substrate for color development. TMB is converted into blue under the catalysis of peroxidase and then into the final yellow under the action of acid. The depth of the color is positively correlated with the hemolytic lecithin acyltransferase 3(LPCAT3) in the sample. The absorbance (O.D. value) is measured at a wavelength of 450nm using an microplate reader, and the sample concentration is calculated. |
| Procedures | 1. Preparation of standards, reagents, and samples before the experiment; 2. Add 100 µL of sample (standard and sample) and incubate at 37°C for 1 hour; 3. Aspirate, add Detection Solution A 100 µL, incubate at 37°C for 1 hour; 4. Wash the plate 3 times; 5. Add 100 µL of Detection Solution B and incubate at 37°C for 30 minutes. 6. Wash the plate 5 times; 7. Add TMB Substrate 90 µL, incubate at 37°C for 10-20 minutes. 8. Add 50 µL of Termination Solution and read at 450 nm immediately. |
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