Cat.No: HTK-HMM-0017 Datasheet
Specification Quantities
| Product Name | Mouse Lysophosphatidylcholine Acyltransferase 2 (LPCAT2) Multiplex Assay Kit-FLIA |
| Catalog No. | HTK-HMM-0017 |
| Description | The primary role of lysophosphatidyl transferase 2 (LPCAT2) is to catalyze the acylation of lysophosphatidylcholine (LPC) to produce lysophosphatidic acid (LPA). LPCAT2 plays a key role in cell membrane phospholipid metabolism and is involved in regulating the fluidity and stability of cell membranes, thereby affecting cell signaling and function. |
| Test Species | Mouse |
| Synonyms | AYTL1; Acyltransferase Like 1; 1-acylglycerol-3-phosphate O-acyltransferase 11; Lysophosphatidic acid acyltransferase alpha; 1-alkylglycerophosphocholine O-acetyltransferase |
| Applications | This kit is used for the detection of hemolytic lecithin phosphatidyl transferase 2 (LPCAT2) and other multi-factors, after testing and other similar substances without significant cross-reactivity. No more than 8 indicators of multi-factors can be mixed in a single test. |
| Sample Type | Tissue homogenates and other biological fluids. |
| Detection Methods | FLIA |
| Estimated Measurement Time | 3.5 h |
| Detection Range | 0.02-20 ng/mL |
| Sensitivity | 0.007 ng/mL |
| Accuracy | Intra-lot variation: CV<10%; Inter-lot variation: CV<12%. |
| Detection Principle | The antibodies of the multi-factor detection kit (flow fluorescence luminescence method) such as lysolytic lecithin acyltransferase 2(LPCAT2), are coated on magnetic beads to prepare solid-phase carriers. Standards or specimens and magnetic beads are added to the microwells, respectively. Among them, the hemolytic lecithin acyltransferase 2(LPCAT2) and other multi-factor detection kits (flow cytometry fluorescence luminescence method) are combined with the antibodies attached to the solid-phase carrier. Then, biotinylated antibodies such as the hemolytic lecithin acyltransferase 2(LPCAT2) and other multi-factor detection kits (flow cytometry fluorescence luminescence method) are added. After washing off the unbound biotinylated antibodies, add the PE-labeled avidin, and after thorough washing again, it can be used for reading on the machine. The MFI value is positively correlated with the multi-factor detection kits such as lysolytic lecithin acyltransferase 2(LPCAT2) in the samples (flow cytometry fluorescence luminescence method). |
| Procedures | 1. Prepare standards, reagents, and samples before the experiment; 2. Add samples (standards, samples, magnetic beads) 100 μL of standards or samples and 10 μL of magnetic beads, and incubate for 90 minutes at 37°C on an enzyme plate oscillator; 3. Drain the plate magnetically, add 100 μL of Detection Solution A, and incubate at 37°C for 60 minutes; 4. Wash the plate 3 times with magnetic suction; 5. Add 100 μL of Detection Solution B and incubate at 37°C for 30 minutes with shaking; 6. Wash the plate 3 times with magnetic suction; 7. Add 100μL of Sheath Solution, vortex for 2 minutes, and read. |
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