Cat.No: HTK-HMM-0003 Datasheet
Specification Quantities
| Product Name | Human Lysophosphatidic Acid Receptor 3 (LPAR3) ELISA Kit |
| Catalog No. | HTK-HMM-0003 |
| Description | The primary intracellular role of lysophosphatidic acid receptor 3 (LPAR3) is to trigger intracellular signaling through members of the G-protein-coupled receptor family, thereby affecting biological behaviors such as cell proliferation, survival, infiltration, and metastasis. |
| Test Species | Human |
| Synonyms | GPCR; EDG7; HOFNH30; LP-A3; LPA3; LPAR3; Endothelial Differentiation, Lysophosphatidic Acid G-Protein-Coupled Receptor 7; Lysophosphatidic acid receptor Edg-7 |
| Applications | This kit is used for the detection of Lysophosphatidic Acid Receptor 3 (LPAR3), there is no obvious cross-reactivity with other similar substances. |
| Sample Type | Tissue homogenates, cell lysates and other biological fluids. |
| Detection Methods | Sandwich ELISA |
| Estimated Measurement Time | 3 h |
| Detection Range | 0.312-20 ng/mL |
| Sensitivity | 0.113 ng/mL |
| Accuracy | Intra-lot variation: CV<10%; Inter-lot variation: CV<12%. |
| Detection Principle | The lysophosphatidic acid receptor 3(LPAR3) antibody is coated in a 96-well microplate to form a solid-phase carrier. Standards or specimens are added to the microplates, respectively, and the lysophosphatidic acid receptor 3(LPAR3) is bound to the antibody attached to the solid-phase carrier. Then, the biotinylated lysophosphatidic acid receptor 3(LPAR3) antibody is added. After washing off the unbound biotinylated antibodies, add HRP-labeled avidin, thoroughly wash again, and then add TMB substrate for color development. TMB is converted into blue under the catalysis of peroxidase and then into the final yellow under the action of acid. The depth of color is positively correlated with lysophosphatidic acid receptor 3(LPAR3) in the sample. The absorbance (O.D. value) is measured at a wavelength of 450nm using an microplate reader, and the sample concentration is calculated. |
| Procedures | 1. Preparation of standards, reagents, and samples before the experiment; 2. Add 100 µL of sample (standard and sample) and incubate at 37°C for 1 hour; 3. Aspirate, add Detection Solution A 100 µL, incubate at 37°C for 1 hour; 4. Wash the plate 3 times; 5. Add 100 µL of Detection Solution B and incubate at 37°C for 30 minutes. 6. Wash the plate 5 times; 7. Add TMB Substrate 90 µL, incubate at 37°C for 10-20 minutes. 8. Add 50 µL of Termination Solution and read at 450 nm immediately. |
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