Cat.No: HTK-HMM-0010 Datasheet
Specification Quantities
| Product Name | Mouse Lysophosphatidylcholine Acyltransferase 1 (LPCAT1) ELISA Kit |
| Catalog No. | HTK-HMM-0010 |
| Description | The main role of lysophosphatidyl transferase 1 (LPCAT1) is to participate in cell membrane remodeling and signal transduction processes by catalyzing the conversion of lysophosphatidylcholine (LPC) to phosphatidylcholine (PC). LPCAT1 plays a key role in cell membrane remodeling by reacylating LPC to PC through the Lands cycle pathway, thereby altering the phospholipid composition of the cell membrane and affecting the structure and function of the cell membrane. |
| Test Species | Mouse |
| Synonyms | AYTL2; PFAAP3; Acyltransferase Like 2; 1-alkylglycerophosphocholine O-acetyltransferase; Phosphonoformate immuno-associated protein 3; Acetyl-CoA:lyso-PAF acetyltransferase |
| Applications | This kit is used for the detection of Lysophosphatidyl transferase 1 (LPCAT1), and there is no significant cross-reactivity with other similar substances. |
| Sample Type | Serum, plasma, tissue homogenates and other biological fluids. |
| Detection Methods | Sandwich ELISA |
| Estimated Measurement Time | 3 h |
| Detection Range | 0.156-10 ng/mL |
| Sensitivity | 0.056 ng/mL |
| Accuracy | Intra-lot variation: CV<10%; Inter-lot variation: CV<12%. |
| Detection Principle | The hemolytic lecithin acyltransferase 1(LPCAT1) antibody is coated in a 96-well microplate to form a solid-phase carrier. Standards or specimens are added to the microplates, respectively, and the hemolytic lecithin acyltransferase 1(LPCAT1) is bound to the antibody attached to the solid-phase carrier. Then, add the biotinylated lysolytic lecithin acyltransferase 1(LPCAT1) antibody. After washing off the unbound biotinylated antibody, add HRP-labeled avidin, thoroughly wash again, and then add TMB substrate for color development. TMB is converted into blue under the catalysis of peroxidase and then into the final yellow under the action of acid. The depth of color is positively correlated with the hemolytic lecithin acyltransferase 1(LPCAT1) in the sample. The absorbance (O.D. value) is measured at a wavelength of 450nm using an microplate reader, and the sample concentration is calculated. |
| Procedures | 1. Preparation of standards, reagents, and samples before the experiment; 2. Add 100 µL of sample (standard and sample) and incubate at 37°C for 1 hour; 3. Aspirate, add Detection Solution A 100 µL, incubate at 37°C for 1 hour; 4. Wash the plate 3 times; 5. Add 100 µL of Detection Solution B and incubate at 37°C for 30 minutes. 6. Wash the plate 5 times; 7. Add TMB Substrate 90 µL, incubate at 37°C for 10-20 minutes. 8. Add 50 µL of Termination Solution and read at 450 nm immediately. |
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