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| Product Name | Masson's Trichrome Stain Kit for Collagen and Muscle Fibers |
| Catalog No. | ATRSA-0025 |
| Description | Modified Masson's trichrome staining kit for differential visualization of collagen (blue/green), muscle fibers (red), cytoplasm (pink), and nuclei (dark brown/black) in tissue sections. The three-color differential staining enables clear identification of fibrotic tissue, collagen deposition, and muscle architecture. Widely used in pathology for liver fibrosis staging, cardiac fibrosis assessment, and tumor stroma evaluation. |
| Intended Use | Differential staining of collagen and muscle in tissue sections; liver fibrosis assessment (METAVIR, Ishak scoring); cardiac fibrosis quantification; kidney fibrosis evaluation; tumor microenvironment stroma analysis; muscle pathology. |
| Principle / Technology | Bouin's or Bouin's-alternative mordant; Weigert's iron hematoxylin stains nuclei; Biebrich scarlet-acid fuchsin stains muscle and cytoplasm red; phosphomolybdic/phosphotungstic acid differentiates; aniline blue or methyl blue stains collagen |
| Detection Method | Deparaffinize and hydrate; mordant in Bouin's solution (56 °C, 1 hr or RT overnight); Weigert's hematoxylin 10 min; Biebrich scarlet-acid fuchsin 5 min; phosphomolybdic-phosphotungstic acid 10 min; aniline blue 5 min; 1% acetic acid 2 min; dehydrate, clear, mount |
| Sample Type | FFPE tissue sections; recommended section thickness 4-5 µm |
| Sensitivity / LOD | Detects collagen fibers at low density; distinguishes mature (dense blue) from immature (pale blue) collagen |
| Specificity | Collagen: blue/green; muscle/cytoplasm: red; nuclei: dark brown/black; elastic fibers: pale pink; mucin: pale blue |
| Reaction Conditions / Protocol | Total protocol ~1.5-2 hr (with Bouin's mordant); microwave-accelerated protocol available (~30 min total) |
| Components / Formulation | Bouin's solution (or Bouin's alternative), Weigert's iron hematoxylin A and B (mix before use), Biebrich scarlet-acid fuchsin solution, phosphomolybdic-phosphotungstic acid solution, aniline blue solution, 1% acetic acid solution, positive control slide (cirrhotic liver or cardiac tissue) |
| Storage Conditions | 15-25 °C; Weigert's components protected from light; Bouin's solution in dark bottle |
| Shelf Life | 12 months; Weigert's working solution stable 2 weeks at RT |
| Package Specifications | Kit for 50 slides, 100 slides |
| Product Form | Liquid reagents; Weigert's A and B components mixed 1:1 before use |
| Key Features | Ready-to-use reagents; Bouin's alternative for picric acid-sensitive labs; both collagen-blue and collagen-green formulations available; Weigert's iron hematoxylin for crisp nuclear detail; includes positive control; validated on automated trainers |
| Purity | All dyes certified by Biological Stain Commission; Weigert's hematoxylin components meet histology grade |
| Concentration | As specified; ready-to-use or concentrate format |
| Activity / Unit Definition | Staining intensity and specificity validated on reference tissue/cell preparations |
| Molecular Weight | Varies by dye component as specified in product documentation |
| Source / Origin | Synthetic dyes and biological stains; certified by Biological Stain Commission where applicable |
| pH Range / Optimal pH | pH 6.0–7.0 for Romanowsky-type stains; varies by formulation |
| Shipping Conditions | Ambient; Bouin's solution contains picric acid — ship as flammable solid when dry; Bouin's alternative ships non-hazardous |
| Expiration Date / Stability | 12 months at 15-25 °C; Bouin's solution should remain yellow — discard if darkens significantly; Weigert's working solution discard if precipitate extensive |
| Regulatory / Compliance | For laboratory and research use only; RUO; Bouin's solution contains picric acid — explosive when dry; ISO 9001 |
| Compatibility | Compatible with FFPE sections from all tissue types; Bouin's-fixed tissues give superior results; formalin-fixed tissues may require Bouin's mordant step for optimal collagen staining; avoid mercuric fixatives which interfere with trichrome staining |
| Recommended Buffer System | Use with provided buffer or phosphate buffer pH 6.8 as specified |
| Application Notes / Precautions | Avoid contact with skin and clothing; stains are permanent; use appropriate PPE |
| Batch-to-Batch Consistency | Staining intensity and differential contrast validated on reference specimens per lot |
For research use only, not for clinical use.
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