| Product Name | Human Glycoprotein VI, Platelet (GP6) ELISA Kit |
| Catalog No. | PAK-HMM-0014 |
| Description | Platelet glycoprotein VI (GP6) is a 58 kD platelet membrane glycoprotein that plays a key role in collagen-induced platelet activation and aggregation. Injury to the vessel wall with subsequent damage to the endothelial lining exposes the subendothelial matrix to blood flow, leading to platelet deposition. Collagen fibers are the most thrombogenic macromolecular component of the extracellular matrix, with collagen types I, III, and VI being the predominant forms in the vasculature. Platelets interact with collagen in two steps. Initial adhesion to collagen followed by an activation step that leads to platelet secretion, recruitment of more platelets, and aggregation. Under physiologic conditions, the resulting platelet plug is the initial hemostatic event that limits blood loss. However, collagen exposure after rupture of an atherosclerotic plaque is a major stimulus for thrombosis associated with myocardial infarction or stroke. |
| Test Species | Human |
| Synonyms | GPIV; GP-VI; Glycoprotein VI, Platelet |
| Applications | The kit detects human GP6 in samples without significant cross-reactivity with its analogs. |
| Sample Type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. |
| Detection Methods | Sandwich ELISA |
| Sensitivity | 26.85 pg/mL |
| Detection Range | 78.12-5000 pg/mL |
| Accuracy | The intraplate coefficient of variation is less than 10% and the interplate coefficient of variation is less than 10%. |
| Storage | 2-8°C |
| Shelf Life | 6 months |
| Detection Principle | The kit is based on enzyme-linked immunosorbent assay (ELISA) using double antibody sandwich method. To the microtiter wells pre-coated with human platelet glycoprotein VI (GP6) capture antibody, the sample, standard, biotin-labeled detection antibody, HRP enzyme conjugate are added sequentially, with warming and washing in the middle, and then the color is developed with the substrate TMB, which is converted to blue by catalysis of peroxidase (HRP), and then converted to the final yellow color under the action of acid. The shade of color is positively correlated with human platelet glycoprotein VI (GP6) in the sample. The absorbance (OD value) is measured at 450 nm with an enzyme marker to calculate the concentration of the sample. |
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