High - Density Lipoprotein Cholesterol (HDL - C) Direct Assay Kit (Clearance Method)
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High - Density Lipoprotein Cholesterol (HDL - C) Direct Assay Kit (Clearance Method)

Cat.No: BLTR-0011 Datasheet

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Product Name High - Density Lipoprotein Cholesterol (HDL - C) Direct Assay Kit (Clearance Method)
Catalog No. BLTR-0011
Description This High - Density Lipoprotein Cholesterol direct assay kit uses the clearance method for direct and specific quantitative detection of HDL - C in serum and plasma. It has excellent precision, low interference, and is helpful for researching HDL - C's role in cardiovascular protection
Test Target High - Density Lipoprotein Cholesterol (HDL - C)
Methodology Direct enzymatic (clearance method)
Measurement Range 0.39-2.59 mmol/L
Sample Type Serum, plasma
Assay Time 10 min at 37°C
Wavelength 600 nm
Linearity 0.39-2.59 mmol/L
Precision (Intra-assay CV) ≤2.5%
Precision (Inter-assay CV) ≤3.5%
Stability (Unopened) 18 months at 2-8°C
Stability (Opened) 30 days at 2-8°C
Stability (Reconstituted) 10 days at 2-8°C
Interference (Hemoglobin) ≤500 mg/dL (no significant interference)
Interference (Bilirubin) ≤20 mg/dL (no significant interference)
Interference (Lipemic) ≤1000 mg/dL (no significant interference)
Reagent Composition Clearance reagents, cholesterol oxidase, peroxidase, 4 - aminoantipyrine
Reagent Form Liquid
Reagent Volume R1: 32 mL×4; R2: 8 mL×4; Cal: 1 mL×1
Calibration Type External calibration
Calibration Levels 4 levels
Incubation Temperature 37°C
Detection Instrument Clinical chemistry analyzer, UV - visible spectrophotometer
Application Quantitative determination of HDL - C in serum/plasma to study its anti - atherosclerotic effects and lipid metabolism regulation
Cross - Reactivity No cross - reactivity with LDL, VLDL
pH Range 6.8-7.2
Shelf Life 18 months
Storage Condition Store at 2-8°C

For research use only, not for clinical use.

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