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| Product Name | Free Fatty Acid Colorimetric Assay Kit |
| Catalog No. | BLTR-0005 |
| Description | This free fatty acid colorimetric assay kit uses the ACS - AC, MCO, POD enzymatic method to accurately quantify free fatty acids in serum, plasma, and cell culture media. It has high sensitivity, a wide linear range, and low interference, supporting research on lipid metabolism and related metabolic disorders |
| Test Target | Free Fatty Acids (FFA) |
| Methodology | Enzymatic colorimetric (ACS - AC, MCO, POD) |
| Measurement Range | 0.05-2 mmol/L |
| Sample Type | Serum, plasma, cell culture medium |
| Assay Time | 10 min at 37°C |
| Wavelength | 550 nm |
| Linearity | 0.05-2 mmol/L |
| Precision (Intra-assay CV) | ≤2% |
| Precision (Inter-assay CV) | ≤3% |
| Stability (Unopened) | 18 months at 2-8°C |
| Stability (Opened) | 30 days at 2-8°C |
| Stability (Reconstituted) | 7 days at 2-8°C |
| Interference (Hemoglobin) | ≤500 mg/dL (no significant interference) |
| Interference (Bilirubin) | ≤20 mg/dL (no significant interference) |
| Interference (Lipemic) | ≤800 mg/dL (no significant interference) |
| Reagent Composition | Acyl - CoA synthetase, acyl - CoA oxidase, malate dehydrogenase, peroxidase, 4 - aminoantipyrine, β - nicotinamide adenine dinucleotide |
| Reagent Form | Liquid |
| Reagent Volume | R1: 50 mL; R2: 10 mL |
| Calibration Type | External calibration |
| Calibration Levels | 3 levels |
| Incubation Temperature | 37°C |
| Detection Instrument | Microplate reader, spectrophotometer |
| Application | Quantitative determination of free fatty acids in biological samples to study lipid metabolism, energy homeostasis, and metabolic diseases |
| Cross - Reactivity | No significant cross - reactivity with triglycerides, cholesterol esters |
| pH Range | 6.5-7.0 |
| Shelf Life | 18 months |
| Storage Condition | Store at 2-8°C, protected from light |
For research use only, not for clinical use.
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