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| Product Name | Cholesterol Esterase Activity Assay Kit |
| Catalog No. | BLTR-0018 |
| Description | This cholesterol esterase activity assay kit uses enzymatic colorimetric method to detect cholesterol esterase activity accurately. It has a wide linear range, good precision, and is suitable for studying cholesterol ester metabolism and enzyme function research |
| Test Target | Cholesterol Esterase Activity |
| Methodology | Enzymatic colorimetric (cholesterol esterase, cholesterol oxidase, peroxidase) |
| Measurement Range | 0.1-10 U/mL |
| Sample Type | Enzyme samples, cell lysates, tissue homogenates |
| Assay Time | 15 min at 37°C |
| Wavelength | 505 nm |
| Linearity | 0.1-10 U/mL |
| Precision (Intra-assay CV) | ≤2.5% |
| Precision (Inter-assay CV) | ≤3.5% |
| Stability (Unopened) | 18 months at 2-8°C |
| Stability (Opened) | 28 days at 2-8°C |
| Stability (Reconstituted) | 10 days at 2-8°C |
| Interference (Hemoglobin) | ≤400 mg/dL (no significant interference) |
| Interference (Bilirubin) | ≤15 mg/dL (no significant interference) |
| Interference (Lipemic) | ≤800 mg/dL (no significant interference) |
| Reagent Composition | Cholesterol ester, cholesterol oxidase, peroxidase, 4 - aminoantipyrine, phenol |
| Reagent Form | Liquid |
| Reagent Volume | R1: 50 mL; R2: 10 mL; Substrate: 5 mL |
| Calibration Type | External calibration |
| Calibration Levels | 3 levels |
| Incubation Temperature | 37°C |
| Detection Instrument | Spectrophotometer, microplate reader |
| Application | Quantitative determination of cholesterol esterase activity in biological samples for research on cholesterol ester metabolism and related enzyme - related functions |
| Cross - Reactivity | No significant cross - reactivity with other esterases |
| pH Range | 6.5-7.0 |
| Shelf Life | 18 months |
| Storage Condition | Store at 2-8°C |
For research use only, not for clinical use.
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