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| Product Name | Glycerol Colorimetric Assay Kit |
| Catalog No. | BLTR-0013 |
| Description | This glycerol colorimetric assay kit adopts enzymatic colorimetric method to accurately quantify glycerol in various biological samples. It has high sensitivity, good linearity, and low interference, supporting research on glycerol - related metabolic pathways and lipid metabolism disorders |
| Test Target | Glycerol |
| Methodology | Enzymatic colorimetric (glycerol kinase, glycerol - 3 - phosphate oxidase, peroxidase) |
| Measurement Range | 0.02-5 mmol/L |
| Sample Type | Serum, plasma, cell culture medium, tissue homogenates |
| Assay Time | 15 min at 37°C |
| Wavelength | 540 nm |
| Linearity | 0.02-5 mmol/L |
| Precision (Intra-assay CV) | ≤2% |
| Precision (Inter-assay CV) | ≤3% |
| Stability (Unopened) | 18 months at 2-8°C |
| Stability (Opened) | 30 days at 2-8°C |
| Stability (Reconstituted) | 10 days at 2-8°C |
| Interference (Hemoglobin) | ≤500 mg/dL (no significant interference) |
| Interference (Bilirubin) | ≤20 mg/dL (no significant interference) |
| Interference (Lipemic) | ≤1000 mg/dL (no significant interference) |
| Reagent Composition | Glycerol kinase, glycerol - 3 - phosphate oxidase, peroxidase, 4 - aminoantipyrine, phenol |
| Reagent Form | Liquid |
| Reagent Volume | R1: 50 mL; R2: 10 mL |
| Calibration Type | External calibration |
| Calibration Levels | 4 levels |
| Incubation Temperature | 37°C |
| Detection Instrument | Microplate reader, spectrophotometer |
| Application | Quantitative determination of glycerol in biological samples to study glycerol metabolism and its role in lipid synthesis and energy supply |
| Cross - Reactivity | No cross - reactivity with glycerol - 3 - phosphate, triglycerides |
| pH Range | 6.8-7.2 |
| Shelf Life | 18 months |
| Storage Condition | Store at 2-8°C |
For research use only, not for clinical use.
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