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| Product Name | Amplex Red Total Cholesterol Assay Kit |
| Catalog No. | BLTR-0012 |
| Description | This Amplex Red total cholesterol assay kit uses a fluorometric method with ultra - high sensitivity, capable of detecting trace total cholesterol in small - volume samples. It has excellent precision and low interference, ideal for in - depth research on cholesterol metabolism in cells and tissues |
| Test Target | Total Cholesterol (TC) |
| Methodology | Fluorometric (Amplex Red, HRP, cholesterol oxidase, cholesterol esterase) |
| Measurement Range | 0.01-10 μM |
| Sample Type | Serum, plasma, cell lysates, tissue homogenates |
| Assay Time | 30 min at 37°C |
| Wavelength | 560 nm (excitation), 590 nm (emission) |
| Linearity | 0.01-10 μM |
| Precision (Intra-assay CV) | ≤1.5% |
| Precision (Inter-assay CV) | ≤2.5% |
| Stability (Unopened) | 24 months at -20°C |
| Stability (Opened) | 60 days at 2-8°C |
| Stability (Reconstituted) | 14 days at 2-8°C |
| Interference (Hemoglobin) | ≤600 mg/dL (no significant interference) |
| Interference (Bilirubin) | ≤25 mg/dL (no significant interference) |
| Interference (Lipemic) | ≤1200 mg/dL (no significant interference) |
| Reagent Composition | Amplex Red reagent, horseradish peroxidase, cholesterol oxidase, cholesterol esterase, buffer |
| Reagent Form | Liquid |
| Reagent Volume | R1: 10 mL; R2: 5 mL; R3: 2 mL |
| Calibration Type | External calibration |
| Calibration Levels | 5 levels |
| Incubation Temperature | 37°C |
| Detection Instrument | Fluorescence microplate reader, fluorometer |
| Application | Quantitative detection of total cholesterol in biological samples for high - sensitivity lipid metabolism research, suitable for small - volume samples |
| Cross - Reactivity | No cross - reactivity with other sterols |
| pH Range | 7.0-7.5 |
| Shelf Life | 24 months |
| Storage Condition | Store unopened at -20°C; opened and reconstituted at 2-8°C, protected from light |
For research use only, not for clinical use.
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