- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Total Cholesterol Colorimetric Assay Kit (Single Reagent COD - PAP Method) |
| Catalog No. | BLTR-0001 |
| Description | This total cholesterol colorimetric assay kit uses the COD - PAP method for accurate and sensitive quantitative detection of total cholesterol in various biological samples. It offers both colorimetric and fluorometric detection options, has a wide linear range, and minimal interference from common substances, making it ideal for lipid metabolism research and cardiovascular disease - related studies |
| Test Target | Total Cholesterol (TC) |
| Methodology | Enzymatic colorimetric (COD - PAP) |
| Measurement Range | 5-500 μM (colorimetric); 0.5-50 μM (fluorometric) |
| Sample Type | Serum, plasma, cell lysates, tissue homogenates |
| Assay Time | 10 min at 37°C |
| Wavelength | 505 nm (colorimetric); 571 nm (excitation), 585 nm (emission) (fluorometric) |
| Linearity | Colorimetric: 5-500 μM; Fluorometric: 0.5-50 μM |
| Precision (Intra-assay CV) | ≤2% |
| Precision (Inter-assay CV) | ≤3% |
| Stability (Unopened) | 18 months at 2-8°C |
| Stability (Opened) | 30 days at 2-8°C |
| Stability (Reconstituted) | 7 days at 2-8°C |
| Interference (Hemoglobin) | ≤500 mg/dL (no significant interference) |
| Interference (Bilirubin) | ≤20 mg/dL (no significant interference) |
| Interference (Lipemic) | ≤1000 mg/dL (no significant interference) |
| Reagent Composition | Cholesterol esterase, cholesterol oxidase, peroxidase, 4 - aminoantipyrine, phenol |
| Reagent Form | Liquid |
| Reagent Volume | R1: 60 mL×4; R2: 20 mL×4 |
| Calibration Type | External calibration |
| Calibration Levels | 4 levels |
| Incubation Temperature | 37°C |
| Detection Instrument | Microplate reader, spectrophotometer |
| Application | Quantitative determination of total cholesterol in biological samples for research on lipid metabolism and cardiovascular disease mechanisms |
| Cross - Reactivity | No significant cross - reactivity with common lipids and biomolecules |
| pH Range | 6.5-7.5 |
| Shelf Life | 18 months |
| Storage Condition | Store unopened at 2-8°C, protected from light; opened reagents and reconstituted solutions should be stored at 2-8°C and used within the specified time |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagenoTech. All rights reserved.