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| Product Name | HDL - Cholesterol Precipitation Assay Kit |
| Catalog No. | BLTR-0003 |
| Description | This HDL - cholesterol precipitation assay kit combines precipitation and enzymatic colorimetric methods to specifically measure HDL - C in serum and plasma. It has high specificity, minimal interference, and a wide linear range, facilitating research on HDL - related lipid metabolism and cardiovascular health |
| Test Target | High - Density Lipoprotein Cholesterol (HDL - C) |
| Methodology | Precipitation (using phosphotungstic acid - magnesium chloride) followed by enzymatic colorimetric (CHOD - PAP) |
| Measurement Range | 0.39-2.59 mmol/L (15-100 mg/dL) |
| Sample Type | Serum, plasma |
| Assay Time | 20 min (precipitation: 10 min; enzymatic reaction: 10 min at 37°C) |
| Wavelength | 505 nm |
| Linearity | 0.39-2.59 mmol/L |
| Precision (Intra-assay CV) | ≤3% |
| Precision (Inter-assay CV) | ≤4% |
| Stability (Unopened) | 24 months at 2-8°C |
| Stability (Opened) | 45 days at 2-8°C |
| Stability (Reconstituted) | 14 days at 2-8°C |
| Interference (Hemoglobin) | ≤400 mg/dL (no significant interference) |
| Interference (Bilirubin) | ≤15 mg/dL (no significant interference) |
| Interference (Lipemic) | ≤800 mg/dL (no significant interference) |
| Reagent Composition | Phosphotungstic acid, magnesium chloride, cholesterol esterase, cholesterol oxidase, peroxidase, 4 - aminoantipyrine |
| Reagent Form | Liquid |
| Reagent Volume | Precipitant: 100 mL; R1: 60 mL×4; R2: 20 mL×4 |
| Calibration Type | External calibration |
| Calibration Levels | 5 levels |
| Incubation Temperature | 37°C (enzymatic reaction) |
| Detection Instrument | Spectrophotometer, clinical chemistry analyzer |
| Application | Quantitative determination of HDL - C in serum/plasma to study its role in reverse cholesterol transport and cardiovascular disease prevention |
| Cross - Reactivity | No cross - reactivity with LDL, VLDL |
| pH Range | 6.8-7.2 |
| Shelf Life | 24 months |
| Storage Condition | Store at 2-8°C, avoid freezing |
For research use only, not for clinical use.
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