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| Product Name | LDL/VLDL Cholesterol Precipitation Assay Kit |
| Catalog No. | BLTR-0015 |
| Description | This LDL/VLDL cholesterol precipitation assay kit combines precipitation and enzymatic colorimetric methods to simultaneously detect LDL - C and VLDL - C. It has high accuracy, good precision, and is suitable for comprehensive lipid metabolism research |
| Test Target | Low - Density Lipoprotein Cholesterol (LDL - C) and Very Low - Density Lipoprotein Cholesterol (VLDL - C) |
| Methodology | Precipitation (heparin - manganese chloride) followed by enzymatic colorimetric |
| Measurement Range | 0.5-13 mmol/L (LDL - C); 0.2-2.0 mmol/L (VLDL - C) |
| Sample Type | Serum, plasma |
| Assay Time | 25 min (precipitation: 15 min; enzymatic reaction: 10 min at 37°C) |
| Wavelength | 505 nm |
| Linearity | 0.5-13 mmol/L (LDL - C); 0.2-2.0 mmol/L (VLDL - C) |
| Precision (Intra-assay CV) | LDL - C: ≤2.5%; VLDL - C: ≤3% |
| Precision (Inter-assay CV) | LDL - C: ≤3.5%; VLDL - C: ≤4% |
| Stability (Unopened) | 18 months at 2-8°C |
| Stability (Opened) | 28 days at 2-8°C |
| Stability (Reconstituted) | 10 days at 2-8°C |
| Interference (Hemoglobin) | ≤500 mg/dL (no significant interference) |
| Interference (Bilirubin) | ≤20 mg/dL (no significant interference) |
| Interference (Lipemic) | ≤1000 mg/dL (no significant interference) |
| Reagent Composition | Heparin, manganese chloride, cholesterol esterase, cholesterol oxidase, peroxidase, 4 - aminoantipyrine |
| Reagent Form | Liquid |
| Reagent Volume | Precipitant: 100 mL; R1: 60 mL; R2: 20 mL |
| Calibration Type | External calibration |
| Calibration Levels | 4 levels |
| Incubation Temperature | 37°C (enzymatic reaction) |
| Detection Instrument | Spectrophotometer, clinical chemistry analyzer |
| Application | Simultaneous quantitative determination of LDL - C and VLDL - C in serum/plasma to study their roles in atherosclerosis and lipid metabolism disorders |
| Cross - Reactivity | No cross - reactivity with HDL - C |
| pH Range | 6.8-7.2 |
| Shelf Life | 18 months |
| Storage Condition | Store at 2-8°C |
For research use only, not for clinical use.
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